Focus Simplexa NATtrol

They represent the preferred industry standard for molecular diagnostic testing and can be used as independent (third party) quality control materials. NATtrol products are prepared from purified microorganisms that are grown in cell culture, in microbial culture, or isolated from the plasma of infected individuals.

NATtrol treatment modifies surface proteins and makes organisms non-infectious and stable in the refrigerator while retaining the complete genome. Inactivation of the organisms is verified by the absence of growth invalidated infectivity tests based on tissue cultures or in validated growth protocols (as appropriate)

  • Non-infectious and stable in the refrigerator
  • Purified and intact organisms
  • Comprehensive process controls to monitor extraction and amplification steps
  • Available in different formats (one or more analytes)
  • Can be used on various molecular assay platforms and analyzes
  • Traceable to WHO international standards (if applicable)
  • Shelf life of 12 to 24 months
  • Extensive coverage of patents * and/or pending patents

Frequent use of NATtrol controls can be used to:

  • Train and supervise laboratory staff
  • Assess lot-to-lot consistency of test kits and test reagents
  • Monitor daily variations in reported results
  • Provide an impartial and independent assessment of the competence of the tests
  • Provide consistent, reliable, and accurate quality control solutions
  • Help identify lab trends

3 COMMON PROBLEMS WHEN DOING AN ELISA

The ELISA immunoassays are among the most versatile to detect, identify and / or quantitate a particular antigen present in our sample. It is a highly sensitive, robust and relatively inexpensive test, quick and easy to carry out.

But despite the apparent simplicity of its protocol, as with most biological assays, there are times when the results are not as expected.

In this post we will focus on  3 common problems when doing an ELISA , and we will try to give some guidelines to be able to  solve them successfully .

Some of the common problems when doing an ELISA are obtaining a weak , low intensity or even non-existent signal , generating a high background noise that distorts the results, or the high inconsistency of the results between wells .

Next we leave you a list of Possible Causes (PC) and Solutions (S) for these 3 common problems when doing an ELISA.

1.- THE SIGNAL INTENSITY IS VERY LOW OR NON-EXISTENT:

PC: Problems with the protocol

S: The essay may not have been prepared correctly, and to fix it it is necessary to review several points:

  • Check that each step of the protocol has been applied correctly
  • Check that the wavelength and filters used in the plate reader are appropriate. For this, the test can be repeated using a positive control.

PC: Antibody problems

S: Antibodies are the most critical reagents in ELISA tests, and it is important to pay attention to the following points:

  • Use the dilution recommended by the manufacturer, or failing that, optimize the dilution of the antibody to be used.
  • Make sure you use enough antibodies, testing different concentrations of the antibodies to identify the optimal concentration for your assay.
  • Make sure that the antibodies have been stored correctly and have not undergone excessive freeze / thaw cycles .

PC: Problems with antigen

S: When the plates are covered with the antigen and when adding the primary antibody the signal is weak or null, there are two main aspects to check:

  • Sometimes it is possible that a sufficient amount of antigen has not adhered to the plate, try adding more.
  • In the case of peptide antigens, they must be previously conjugated to a large carrier protein in order to facilitate the recognition of the epitope by the antibody.

PC: Problems with reagents

S: Always make sure that:

  • The reagents are at room temperature before starting the assay
  • Check expiration dates
  • Check that they have been prepared correctly and have been added in the corresponding order.
  • Make sure that they do not interfere with other buffers or compounds present in the sample such as sodium acid, EDTA, etc.
  • Avoid mixing reagents from different kits.

PC: Low incubation times and temperature

S: Be sure to incubate the samples for the time specified by the manufacturer and optimize the incubation temperature for your assay. Remember that the reagents must be at room temperature before starting the assay.

PC: Storage conditions

S: Stores reagents to your specifications, keeping in mind that all reagents may not need the same storage requirements.

2.- THE BACKGROUND NOISE IS VERY HIGH

PC: Antibody problems

S: Regarding antibodies, there can be several cases:

  • That the concentration of antibody used is too high. This will be resolved by reducing its concentration, or by carrying out an optimization test to determine the ideal concentration.
  • There are cross reactivity problems, for which a negative control should be used.
  • Non-specific binding where the antibody is detecting other molecules besides the target antigen. The solution is through the use of affinity purified antibodies and the use of the appropriate blocking buffers .

PC: Problems with reagents

S: Regarding the different reagents, we must make sure that:

  • The stop solution is added to avoid an over development of the reaction.
  • The detection reagent has been properly diluted, and if so, retest with an even higher dilution.
  • The appropriate blocking buffers have been used and the blocking time has been sufficient.

PC: Incubation problems

S: In case of obtaining high background noise, it may be necessary to reduce the incubation temperature. On the other hand, it is necessary to ensure that the incubation of the substrate is carried out in dark conditions.

PC: Insufficient washes

S: Carefully wash the bottom of the plate and repeat the reading.

3.- THE VARIATION OF RESULTS BETWEEN WELLS IS VERY HIGH

PC: Inadequate incubation

S: Regarding the incubation step, it is important:

  • Do not stack the plates during the process to ensure that the temperature is evenly distributed.
  • Make sure there are no bubbles inside the wells before incubation.

PC: improper washing

S: Make sure to thoroughly wash all wells, as inhomogeneous washing could result in a high coefficient of variation.

 

 

How To Improve Protein Quality

When working with protein samples , there are critical factors that must be closely monitored to avoid their degradation or dysfunction and guarantee the reliability of the results.

These factors therefore reflect the quality of the proteins and can be basically summarized as:

  • Integrity
  • Purity
  • Homogeneity
  • Solubility
  • Stability
  • Storage

In this post we bring you some tips to improve the quality of proteins and thus optimize the results of the tests.

1.- OPTIMIZATION OF PROTEIN PURITY AND INTEGRITY

Optimizing the purity and integrity of proteins involves avoiding or minimizing contamination of the sample with impurities, modified proteins or degradation products, among others.

Some of the steps that can be taken to this end are:

  1. Change the purification protocol by modifying for example the washing and elution conditions or adding additional purification steps such as ion exchange chromatography.
  2. Modify the conditions of induction of protein expression
  3. Use a different cloning vector
  4. Use a different expression system

2.- OPTIMIZATION OF PROTEIN HOMOGENEITY AND SOLUBILITY

To prevent or eliminate the formation of aggregates and increase the solubility of the protein s interest, there are several points that can affect, such as:

  1. Always perform size exclusion chromatography as the last step in the protein purification process.
  2. Do not over-concentrate the sample, since the processes applied for this purpose usually induce the aggregation of proteins (Remember this entry about 5 techniques to concentrate proteins ).
  3. Modify the composition of the buffer in which the protein is located until optimizing the pH, salinity, additives, etc. best suited in each case.

3.- OPTIMIZATION OF THE STABILITY AND STORAGE OF PROTEINS

Regarding the stability and medium or long-term storage of proteins, it is common to think that freezing and cryopreservation is the ideal method. And although this is true in a large part of the cases, it is not always the case due to the intrinsic variability of each individual protein.

That is why it is advisable to study the stability of each protein of interest to identify the optimal storage method in each case, whether it be simple refrigeration, freezing, lyophilization, etc.

You can expand the information in this post on How to store proteins to avoid their degradation .

 

 

A novel isolator-based system promotes viability of human embryos during laboratory processing.

A novel isolator-based system promotes viability of human embryos during laboratory processing.

In vitro fertilisation (IVF) and associated applied sciences are arguably essentially the most difficult of all cell tradition purposes. The beginning materials is a single cell from which one goals to supply an embryo succesful of establishing a being pregnant ultimately resulting in a dwell beginning.

Laboratory processing during IVF remedy requires open manipulations of gametes and embryos, which usually entails publicity to ambient situations.

To scale back the danger of mobile stress, we’ve got developed a very enclosed system of interlinked isolator-based workstations designed to keep up oocytes and embryos in a physiological setting all through the IVF course of. Comparison of scientific and laboratory information earlier than and after the introduction of the brand new system revealed that considerably extra embryos developed to the blastocyst stage within the enclosed isolator-based system in contrast with standard open-fronted laminar circulate hoods.

Moreover, blastocysts produced within the isolator-based system contained considerably extra cells and their growth was accelerated. Consistent with this, the introduction of the enclosed system was accompanied by a major enhance within the scientific being pregnant fee and within the proportion of embryos implanting following switch to the uterus.

alpha Tubulin (alpha Tubulin) Antibody

abx230333-100ug 100 ug
EUR 610.8

alpha Tubulin (alpha Tubulin) Antibody

abx230334-100ug 100 ug
EUR 610.8

alpha Tubulin (alpha Tubulin) Antibody

abx230335-100ug 100 ug
EUR 610.8

alpha Tubulin (alpha tubulin) Antibody

20-abx133573
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 30 ul

alpha Tubulin (alpha tubulin) Antibody

20-abx134672
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 30 ul

alpha Tubulin (alpha tubulin) Antibody

20-abx134674
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 30 ul

alpha Tubulin (alpha Tubulin) Antibody

abx005570-50ul 50 ul
EUR 326.4

alpha Tubulin (alpha Tubulin) Antibody

20-abx005573
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 50 ul

alpha Tubulin (alpha Tubulin) Antibody

abx019012-100ug 100 ug
EUR 385.2

Rabbit anti Arabidopsis thaliana Tubulin alpha-1 chain (TUBA1) IgG affinity pure, loading control for WB

TUBA111-A 100 ug
EUR 548.4

alpha Tubulin (alpha Tubulin) Antibody (ALP)

abx446849-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (APC)

abx446850-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (HRP)

abx446853-100ug 100 ug
EUR 678

alpha Tubulin (alpha Tubulin) Antibody (RPE)

abx446856-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (FITC)

abx446852-100ug 100 ug
EUR 678

alpha Tubulin (alpha Tubulin) Antibody (PerCP)

abx446855-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (Biotin)

abx446851-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (Streptavidin)

abx446857-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (ATTO 390)

abx446841-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (ATTO 488)

abx446842-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (ATTO 565)

abx446843-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (ATTO 594)

abx446844-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (ATTO 633)

abx446845-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (ATTO 655)

abx446846-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (ATTO 680)

abx446847-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (ATTO 700)

abx446848-100ug 100 ug
EUR 693.6

alpha Tubulin (alpha Tubulin) Antibody (PE/ATTO 594)

abx446854-100ug 100 ug
EUR 710.4

Alpha Tubulin

E8RE6006 100ul
EUR 275
Description: Available in various conjugation types.

alpha Tubulin 4A

E8ET1705-31 100ul
EUR 275
Description: Available in various conjugation types.

Rabbit Polyclonal antibody to alpha Tubulin (tubulin, alpha 1b)

TA308986 100 µl Ask for price

Rabbit Anti-Bovine tubulin antiserum

TUBL15-S 100 ul
EUR 548.4

Rabbit Polyclonal antibody to alpha Tubulin 1A (tubulin, alpha 1a)

TA307898 100 µl Ask for price

Rabbit Polyclonal antibody to alpha Tubulin 1A (tubulin, alpha 1a)

TA307921 100 µl Ask for price

alpha Tubulin antibody

10C-CR2063M1 100 ul
EUR 95
Description: Mouse monoclonal alpha Tubulin antibody

alpha Tubulin antibody

10R-6523 100 ug
EUR 248
Description: Mouse monoclonal alpha Tubulin antibody

alpha Tubulin antibody

10R-7916 100 ug
EUR 519
Description: Mouse monoclonal alpha Tubulin antibody

alpha Tubulin antibody

10R-T130a 100 ug Ask for price
Description: Mouse monoclonal alpha Tubulin antibody

alpha Tubulin antibody

10R-8421 100 ul
EUR 471.6
Description: Mouse monoclonal alpha Tubulin antibody

alpha Tubulin antibody

20R-2948 100 ul
EUR 471.6
Description: Rabbit polyclonal alpha Tubulin antibody

alpha Tubulin antibody

22038 100ul
EUR 479

alpha Tubulin antibody

22038-100ul 100ul
EUR 468

alpha Tubulin Antibody

E11-184651 100ug/100ul
EUR 225
Description: Available in various conjugation types.

alpha Tubulin Antibody

GWB-266FC4 0.1 mg Ask for price

alpha Tubulin Antibody

GWB-2E674F 0.1 mg Ask for price

alpha Tubulin Antibody

GWB-3092B9 0.5 ml Ask for price

alpha Tubulin antibody

E39-00333 100ug/100ul
EUR 225
Description: Available in various conjugation types.

alpha Tubulin Antibody

AF4651 200ul
EUR 540

alpha Tubulin Antibody

AF4651-100ul 100ul
EUR 350

alpha Tubulin Antibody

AF4651-200ul 200ul
EUR 450

alpha Tubulin Antibody

AF4651-50ul 50ul
EUR 250

alpha Tubulin antibody

70R-13229 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal alpha Tubulin antibody

alpha Tubulin antibody

CAF50026-100ug 100ug
EUR 338

alpha Tubulin Antibody

GWB-75FBD1 0.1 ml Ask for price

Alpha Tubulin Antibody

R30095 100 ug
EUR 356.15
Description: Alpha-tubulin (b-alpha-1) mRNA is expressed at a molecular weight of about 50-55,000D. The 3-prime UTR of the protein is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene IL-alpha-T1. Alpha tubulin is a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.

Tubulin alpha Antibody

E18-7010-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Tubulin alpha Antibody

E18-7010-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Tubulin alpha Antibody

E18-0524-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Tubulin alpha Antibody

E18-0524-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Tubulin alpha Antibody

GWB-1C3320 0.1 mg Ask for price

Tubulin alpha Antibody

AF0524 200ul
EUR 420

Tubulin alpha Antibody

AF0524-100ul 100ul
EUR 280

Tubulin alpha Antibody

AF0524-200ul 200ul
EUR 350

Tubulin alpha Antibody

AF7010 1ml
EUR 1440

Tubulin alpha Antibody

AF7010-100ul 100ul
EUR 250

Tubulin alpha Antibody

AF7010-1ml 1ml
EUR 1200

Tubulin alpha Antibody

AF7010-200ul 200ul
EUR 350

Tubulin alpha Antibody

AF7010-50ul 50ul
EUR 150

Tubulin alpha antibody

70R-30819 100 ug
EUR 294
Description: Rabbit polyclonal Tubulin alpha antibody

Tubulin alpha Antibody

20-abx013239
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Tubulin alpha Antibody

ABF7010 100 ug
EUR 525.6

Tubulin alpha Antibody

ABF0524 100 ug
EUR 525.6

Tubulin Alpha Antibody

GWB-80936D 0.5 mg Ask for price

Tubulin Alpha Antibody

GWB-CEDDB2 0.5 mg Ask for price

Tubulin alpha Antibody

T0033 1ml
EUR 1440

Tubulin alpha Antibody

T0033-100ul 100ul
EUR 250

Tubulin alpha Antibody

T0033-1ml 1ml
EUR 1200

Tubulin alpha Antibody

T0033-200ul 200ul
EUR 350

Tubulin alpha Antibody

T0033-50ul 50ul
EUR 150

Tubulin alpha Antibody

abx013239-100g 100 µg
EUR 237.5

Tubulin alpha Antibody

abx013239-10g 10 µg
EUR 43.75

Tubulin alpha Antibody

abx013239-200g 200 µg
EUR 312.5

alpha Tubulin Mouse mAb

E2222034 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

alpha Tubulin Rabbit pAb

E2380636 100ul
EUR 225
Description: Available in various conjugation types.

alpha Tubulin Rabbit pAb

E2380637 100ul
EUR 225
Description: Available in various conjugation types.

alpha Tubulin Rabbit pAb

E2619725 100ul
EUR 225
Description: Available in various conjugation types.

alpha Tubulin Rabbit mAb

E2R23452 100μl
EUR 225
Description: Available in various conjugation types.

alpha Tubulin Rabbit mAb

E2R23453 100ul
EUR 255
Description: Available in various conjugation types.

alpha Tubulin Rabbit mAb

E2R23454 100ul
EUR 255
Description: Available in various conjugation types.

alpha Tubulin Rabbit pAb

E2540004 100ul
EUR 225
Description: Available in various conjugation types.

alpha Tubulin Rabbit mAb

AC013 50 ul
EUR 318

alpha Tubulin Rabbit mAb

58503 100ul
EUR 339

alpha Tubulin Rabbit mAb

58504 100ul
EUR 339

alpha Tubulin 4a antibody

22537 100ul
EUR 479

alpha Tubulin 4a antibody

22537-100ul 100ul
EUR 468

alpha Tubulin 4A Antibody

E19-2901-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

alpha Tubulin 4A Antibody

E19-2901-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

alpha Tubulin 4A Antibody

DF2901 200ul
EUR 420

alpha Tubulin 4A Antibody

49624 100ul
EUR 499

alpha Tubulin 4A Antibody

49624-100ul 100ul
EUR 399.6

alpha Tubulin 4A Antibody

49624-50ul 50ul
EUR 286.8

alpha Tubulin 1A antibody

70R-13312 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal alpha Tubulin 1A antibody

alpha Tubulin 4a antibody

70R-13517 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal alpha Tubulin 4a antibody

alpha Tubulin 1A antibody

70R-13631 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal alpha Tubulin 1A antibody

alpha Tubulin 4A antibody

70R-2911 50 ug
EUR 467
Description: Rabbit polyclonal alpha Tubulin 4A antibody raised against the middle region of TUBA4A

alpha Tubulin 3C antibody

70R-2123 50 ug
EUR 467
Description: Rabbit polyclonal alpha Tubulin 3C antibody raised against the N terminal of TUBA3C

alpha Tubulin 3C antibody

70R-2135 50 ug
EUR 467
Description: Rabbit polyclonal alpha Tubulin 3C antibody raised against the N terminal of TUBA3C

alpha Tubulin 4A antibody

70R-2151 50 ug
EUR 467
Description: Rabbit polyclonal alpha Tubulin 4A antibody raised against the middle region of TUBA4A

alpha Tubulin 4A Antibody

ABD2901 100 ug
EUR 525.6

alpha Tubulin 4A Antibody

abx148144-100ug 100 ug
EUR 526.8

Alpha Tubulin Antibody / TUB1

RQ5287 100ul
EUR 356.15
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt]

Alpha Tubulin Antibody / TUBA

V8404-100UG 100 ug
EUR 349.3
Description: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. This MAb recognizes an epitope of alpha-tubulin. The alpha-tubulin is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain

Alpha Tubulin Antibody / TUBA

V8404-20UG 20 ug
EUR 153.3
Description: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. This MAb recognizes an epitope of alpha-tubulin. The alpha-tubulin is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain

Alpha Tubulin Antibody / TUBA

V8404SAF-100UG 100 ug
EUR 349.3
Description: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. This MAb recognizes an epitope of alpha-tubulin. The alpha-tubulin is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain

Monoclonal Anti-Human Gamma Tubulin peptide IgG

TUBG31-A 100 ul
EUR 578.4

Tubulin alpha-1A chain

AP88267 1mg
EUR 2640

Tubulin alpha-1A chain

AP88381 1mg
EUR 2640

Tubulin alpha-1A chain

AP88906 1mg
EUR 2640

anti- alpha Tubulin antibody

FNab00332 100µg
EUR 658.5
Description: Antibody raised against alpha Tubulin

anti- alpha Tubulin antibody

FNab00333 100µg
EUR 658.5
Description: Antibody raised against alpha Tubulin

anti- alpha Tubulin antibody

FNab00334 100µg
EUR 658.5
Description: Antibody raised against alpha Tubulin

anti- alpha Tubulin antibody

FNab00335 100µg
EUR 658.5
Description: Antibody raised against alpha Tubulin

alpha Tubulin Antibody (FITC)

GWB-3975FE 0.1 mg Ask for price

Human tubulin, beta 1 (TUBB1) Control/blocking peptide

AB-23096-P 100ug
EUR 196.8

Monoclonal Anti-Rat beta 3-Tubulin peptide IgG

TUBB21-M 100 ul
EUR 578.4

Alpha Tubulin Antibody / TUBA1A

RQ5776 100 ug
EUR 356.15
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Alpha Tubulin Antibody / TUBA1B

R20196-0.1ML 100ul
EUR 347.65
Description: This recombinant Alpha Tubulin antibody reacts to a-Tubulin, including Tubulin alpha-1A chain and Tubulin alpha-1B chain.

Alpha Tubulin Antibody / TUBA1B

R20196BTN-50UL 50ul
EUR 347.65
Description: This recombinant Alpha Tubulin antibody reacts to a-Tubulin (1A & 1B).

Alpha Tubulin (TUBA1B) Antibody

abx005573-100l 100 µl
EUR 212.5

Alpha Tubulin (TUBA1B) Antibody

abx230332-100g 100 µg
EUR 350

Alpha Tubulin (TUBA1B) Antibody

abx230333-100g 100 µg
EUR 350

Alpha Tubulin (TUBA1B) Antibody

abx230334-100g 100 µg
EUR 350

Alpha Tubulin (TUBA1B) Antibody

abx230335-100g 100 µg
EUR 350

Alpha Tubulin (TUBA1B) Antibody

abx019012-1mg 1 mg
EUR 243.75

The information point out that safety from ambient situations promotes improved growth of human embryos. Importantly, we discovered that it was solely possible to conduct all IVF-related procedures within the isolator-based workstations.

A novel isolator-based system promotes viability of human embryos during laboratory processing.

A novel isolator-based system promotes viability of human embryos during laboratory processing.

A clinician’s private view of assisted reproductive know-how over 35 years.

This invited presentation is meant to cowl scientific developments within the evolution of assisted reproductive know-how (ART), a course of which was tried during the 1940’s and 50’s and culminated within the first fruition in 1978. The first in vitro fertilisation (IVF) youngster ensued following the partnership by a scientist with a focussed ambition (Nobel laureate Robert Edwards) becoming a member of with the gynaecologist who launched laparoscopy to Britain within the late 60’s (Patrick Steptoe).

My journey commenced in 1976 as a clinician who grew to become immersed within the embryological and endocrinological science, whence most progress in ART emanates, and continued right into a medical directorship place from which this private view is documented.

Several scientific advances have been essential developments within the understanding and administration of sub-fertile sufferers. However evolution of the assorted laboratory sciences has been the foremost key important to assembly each the rapid in addition to the long-term wants for human replica.

The future requires a a lot better understanding and management over gametogenesis and a laboratory course of which way more carefully duplicates intrinsic reproductive physiology, avoiding gamete and embryo publicity to the ambiance.

This invited presentation is meant to cowl scientific developments within the evolution of assisted reproductive know-how (ART), a course of which was tried during the 1940’s and 50’s and culminated within the first fruition in 1978. The first in vitro fertilisation (IVF) youngster ensued following the partnership by a scientist with a focussed ambition (Nobel laureate Robert Edwards) becoming a member of with the gynaecologist who launched laparoscopy to Britain within the late 60’s (Patrick Steptoe).

My journey commenced in 1976 as a clinician who grew to become immersed within the embryological and endocrinological science, whence most progress in ART emanates, and continued right into a medical directorship place from which this private view is documented. Several scientific advances have been essential developments within the understanding and administration of sub-fertile sufferers.

However evolution of the assorted laboratory sciences has been the foremost key important to assembly each the rapid in addition to the long-term wants for human replica. The future requires a a lot better understanding and management over gametogenesis and a laboratory course of which way more carefully duplicates intrinsic reproductive physiology, avoiding gamete and embryo publicity to the ambiance.

On the fate of primordial germ cells injected into early mouse embryos.

On the fate of primordial germ cells injected into early mouse embryos.

Primordial germ cells (PGCs) are the founder cells of the germline. Via gametogenesis and fertilisation this lineage generates a brand new embryo in the subsequent technology. PGCs are additionally the cell of origin of multilineage teratocarcinomas.

In vitro, mouse PGCs can provide rise to embryonic germ (EG) cells – pluripotent stem cells that may contribute to main chimaeras when launched into pre-implantation embryos. Thus, PGCs can provide rise to pluripotent cells in the course of the developmental cycle, throughout teratocarcinogenesis and by in vitro tradition. However, there isn’t any proof that PGCs can differentiate instantly into somatic cell varieties.

Furthermore, it’s typically assumed that PGCs don’t contribute to chimaeras following injection into the early mouse embryo. However, these knowledge have by no means been formally revealed. Here, we current the main knowledge from the unique PGC-injection experiments carried out 40 years in the past, alongside outcomes from newer research in three separate laboratories.

These outcomes have knowledgeable and influenced present fashions of the relationship between pluripotency and the germline cycle. Current applied sciences enable additional experiments to substantiate and develop upon these findings and permit definitive conclusions as to the developmental efficiency of PGCs.

On the fate of primordial germ cells injected into early mouse embryos.

On the fate of primordial germ cells injected into early mouse embryos.

MC Chang–Reproductive biologist of distinction 1908-1991.

This article evaluations the outstanding life and main scientific achievements of the reproductive biologist M.C. Chang. His scholarly profession progressed from college in Peking, through Edinburgh, Scotland, and Cambridge, England, to the newly based Worcester Foundation for Experimental Biology in Massachusetts. At every stage, the hand of fate is famous as are the help and encouragement of key professors.

Chang’s personal contributions on capacitation of spermatozoa, in vitro fertilisation of mammalian eggs, and transplantation of oocytes and embryos are all introduced out, as is his important enter to the creation and improvement of a steroid contraceptive capsule.

TCR alpha / beta Antibody

abx140255-01mg 0.1 mg
EUR 376.8

TCR alpha + beta Antibody

GWB-52CC1E 0.125 mg Ask for price

TCR alpha + beta Antibody

GWB-126D6D 0.5 mg Ask for price

TCR alpha + beta Antibody

GWB-197296 0.125 mg Ask for price

TCR alpha + beta Antibody

GWB-E5A37C 0.25 mg Ask for price

TCR alpha / beta Antibody

abx139894-100g 100 µg
EUR 275

TCR alpha / beta Antibody

abx140255-100g 100 µg
EUR 237.5

alpha beta TcR antibody (biotin)

61R-1695 100 ug
EUR 415
Description: Mouse monoclonal alpha beta TcR antibody (biotin)

TCR alpha / beta Antibody (PE)

abx139897-100tests 100 tests
EUR 577.2

TCR alpha / beta Antibody (PE)

abx140308-01mg 0.1 mg
EUR 510

TCR alpha + beta Antibody (PE)

GWB-A6B3AD 0.05 mg Ask for price

TCR alpha / beta Antibody (PE)

abx139897-100g 100 µg
EUR 400

TCR alpha / beta Antibody (PE)

abx140308-100g 100 µg
EUR 350

TCR alpha / beta Antibody (APC)

abx139898-100tests 100 tests
EUR 577.2

Rat TCR Alpha/Beta Antibody

GWB-E07536 0.1 mg Ask for price

Rat TCR Alpha/Beta Antibody

GWB-D6710C 0.25 mg Ask for price

TCR alpha / beta Antibody (APC)

abx139898-100g 100 µg
EUR 400

TCR alpha / beta Antibody (FITC)

abx139900-100tests 100 tests
EUR 510

TCR alpha / beta Antibody (FITC)

abx140307-01mg 0.1 mg
EUR 477.6

TCR alpha/beta Antibody (FITC)

GWB-1989AB 100 TESTS/2ml Ask for price

TCR alpha + beta Antibody (FITC)

GWB-1EA05C 0.1 mg Ask for price

TCR alpha + beta Antibody (FITC)

GWB-9A0020 0.05 mg Ask for price

TCR alpha / beta Antibody (FITC)

abx139900-100g 100 µg
EUR 350

TCR alpha / beta Antibody (FITC)

abx140307-100g 100 µg
EUR 325

TCR alpha / beta Antibody (PerCP)

abx139896-100tests 100 tests
EUR 577.2

TCR alpha / beta Antibody (PerCP)

abx139896-100g 100 µg
EUR 400

TCR alpha / beta Antibody (Biotin)

abx139899-01mg 0.1 mg
EUR 510

TCR alpha + beta Antibody (Biotin)

GWB-E48252 0.1 mg Ask for price

TCR alpha + beta Antibody (Biotin)

GWB-D5896D 50 TESTS Ask for price

TCR alpha / beta Antibody (Biotin)

abx139899-100g 100 µg
EUR 350

T-Cel Receptor alpha/beta (TCR alpha/beta) Antibody

abx140619-100g 100 µg
EUR 275

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody

abx413042-025mg 0.25 mg
EUR 678

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody

abx414734-05mg 0.5 mg
EUR 994.8

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody

abx414736-025mg 0.25 mg
EUR 678

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody

abx414737-01mg 0.1 mg
EUR 526.8

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody

abx413042-200l 200 µl
EUR 462.5

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody

abx414734-200l 200 µl
EUR 775

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody

abx414736-200l 200 µl
EUR 487.5

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody

abx414737-200l 200 µl
EUR 350

Mouse TCR Antibody (alpha/beta beta chain)

GWB-26E89F 0.02 mg Ask for price

Mouse TCR Antibody (alpha/beta beta chain)

GWB-D901EB 0.25 mg Ask for price

Anti-Hu TCR alpha/beta PE

1P-607-T025 25 tests
EUR 121

Anti-Hu TCR alpha/beta PE

1P-607-T100 100 tests
EUR 242

Anti-Rt TCR alpha/beta PE

1P-653-C025 0.025 mg
EUR 99

Anti-Rt TCR alpha/beta PE

1P-653-C100 0.1 mg
EUR 198

Anti-Hu TCR alpha/beta APC

1A-607-T025 25 tests
EUR 121

Anti-Hu TCR alpha/beta APC

1A-607-T100 100 tests
EUR 242

Anti-Hu TCR alpha/beta FITC

1F-607-T025 25 tests
EUR 99

Anti-Hu TCR alpha/beta FITC

1F-607-T100 100 tests
EUR 198

Anti-Rt TCR alpha/beta FITC

1F-653-C025 0.025 mg
EUR 88

Anti-Rt TCR alpha/beta FITC

1F-653-C100 0.1 mg
EUR 176

Anti-Hu TCR alpha/beta PerCP

PC-607-T025 25 tests
EUR 121

Anti-Hu TCR alpha/beta PerCP

PC-607-T100 100 tests
EUR 242

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody (FITC)

abx413043-01mg 0.1 mg
EUR 610.8

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody (FITC)

abx413044-05mg 0.5 mg
EUR 861.6

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody (FITC)

abx414735-01mg 0.1 mg
EUR 610.8

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody (FITC)

abx413043-200l 200 µl
EUR 400

T-Cell Receptor Alpha Beta (TCR Alpha/Beta) Antibody (FITC)

abx414735-200l 200 µl
EUR 425

Anti-Hu TCR alpha/beta Biotin

1B-607-C025 0.025 mg
EUR 99

Anti-Hu TCR alpha/beta Biotin

1B-607-C100 0.1 mg
EUR 198

Rat TCR alpha/beta Antibody (RPE)

GWB-D35473 100 TESTS Ask for price

Rat TCR alpha/beta Antibody (FITC)

GWB-2E2AC4 0.1 mg Ask for price

Anti-Hu TCR alpha/beta Purified

11-607-C025 0.025 mg
EUR 71.5

Anti-Hu TCR alpha/beta Purified

11-607-C100 0.1 mg
EUR 143

Anti-Rt TCR alpha/beta Purified

11-653-C100 0.1 mg
EUR 110

Rat TCR alpha/beta Antibody (Biotin)

GWB-2EF868 0.1 mg Ask for price

Chicken TCR alpha/beta Antibody (Vb1)

GWB-Q00908 0.25 mg Ask for price

Chicken TCR alpha/beta Antibody (Vb2)

GWB-Q00909 0.25 mg Ask for price

Rabbit Anti-Human p73 alpha/beta antiserum # 1

P73A11-S 100 ul
EUR 548.4

Human p73 alpha/beta Control/blocking peptide #1

P73A11-P 100 ug
EUR 196.8

Alpha V Beta 5 Integrin, Peptide Aptamer, unlabeled

AP-301-U 5 mg Ask for price

Actin (Pan, alpha, beta, gamma) Control/blocking peptide

ACTB22-P 100 ug
EUR 196.8

T-Cell Receptor Alpha Beta V Beta 1 (TCR Alpha/Beta Vb1) Antibody

abx415177-025mg 0.25 mg
EUR 710.4

T-Cell Receptor Alpha Beta V Beta 2 (TCR Alpha/Beta Vb2) Antibody

abx415178-025mg 0.25 mg
EUR 710.4

T-Cell Receptor Alpha Beta V Beta 1 (TCR Alpha/Beta Vb1) Antibody

abx415177-200l 200 µl
EUR 512.5

T-Cell Receptor Alpha Beta V Beta 2 (TCR Alpha/Beta Vb2) Antibody

abx415178-200l 200 µl
EUR 512.5

OASA04255-100UG - TCR ALPHA/BETA Antibody

OASA04255-100UG 0.1mg
EUR 309

Alpha V Beta 5 Integrin, Peptide Aptamer, Biotinylated

AP-301-B 1 mg Ask for price

Rabbit Anti-Human p73 alpha/beta IgG # 1, aff pure

P73A11-A 100 ug
EUR 578.4

Alpha V Beta 5 Integrin, Peptide Aptamer, FITC labelled

AP-301-F 1 mg Ask for price

Rabbit Anti-Actin (Pan, alpha, beta, gamma) IgG, aff pure

ACTB22-A 100 ug
EUR 578.4

Mouse TCR Antibody (alpha/beta chain) (RPE)

GWB-AE1B68 25 TESTS Ask for price

Mouse Monoclonal Anti-Actin (Pan, alpha, beta, gamma)-biotinylated

ACTB24-BTN 50 ul
EUR 489.6

Mouse TCR Antibody (alpha/beta chain) (FITC)

GWB-818EA7 0.1 mg Ask for price

Mouse TCR Antibody (alpha/beta chain) (FITC)

GWB-CA045D 0.02 mg Ask for price

Mouse TCR Antibody (alpha/beta chain) (FITC)

GWB-CFE6C9 0.5 mg Ask for price

Rat Tcr Alpha/Beta (low endotoxin) Antibody

GWB-51A69B 0.5 mg Ask for price

Mouse TCR Antibody (alpha/beta chain) (Biotin)

GWB-1DC378 0.1 mg Ask for price

Anti-Hu TCR alpha/beta PE-Cy™5

T8-607-T100 100tests
EUR 242

Mouse Monoclonal Anti-Actin (Pan, alpha, beta, gamma) IgG, aff pure

ACTB24-M 100 ug
EUR 578.4

Monoclonal Anti-Human/mouse/rat HSP90 alpha/beta IgG, aff pure

HSP901-M 100 ul
EUR 578.4

OASA04254-250UG - Rat TCR ALPHA/BETA Antibody

OASA04254-250UG 0.25mg
EUR 459

OASA04258-500UG - Rat TCR ALPHA/BETA Antibody

OASA04258-500UG 0.5mg
EUR 699

Mouse Monoclonal Anti-Actin (Pan, alpha, beta, gamma) IgG-Cy3 conjugate

ACTB24-Cy3 100 ul
EUR 489.6

Mouse Monoclonal Anti-Actin (Pan, alpha, beta, gamma) IgG-FITC conjugate

ACTB24-FITC 100 ul
EUR 489.6

Endothelial intergerin Alpha-V Beta-3 (Clone 17.16), RNA Aptamer, unlabeled

AR-287-U Custom Ask for price

Anti-Rt TCR alpha/beta Purified Low Endotoxin

12-653-C100 0.1mg
EUR 143

OASA00155-250UG - Hamster ANTI TCR ALPHA/BETA BETA CHAIN Antibody

OASA00155-250UG 0.25mg
EUR 459

Pig Anti-C. perfringens toxins (Alpha+beta+Epsilon) Antibody ELISA, 96 tests, Quantitative

RB-500720-1 100 ul
EUR 854.4

Dog Anti-C. perfringens toxins (Alpha+beta+Epsilon) Antibody ELISA, 96 tests, Quantitative

RB-500760-1 100 ul
EUR 854.4

Mouse Anti-C. perfringens toxins (Alpha+beta+Epsilon) Antibody ELISA, 96 tests, Quantitative

RB-500710-1 100 ul
EUR 854.4

Rabbit Anti-C. perfringens toxins (Alpha+beta+Epsilon) Antibody ELISA, 96 tests, Quantitative

RB-500700-1 100 ul
EUR 854.4

Chicken Anti-C. perfringens toxins (Alpha+beta+Epsilon) Antibody ELISA, 96 tests, Quantitative

RB-500750-1 100 ul
EUR 854.4

OASA04257-100UG - TCR Alpha/Beta Antibody - FITC Conjugated

OASA04257-100UG 0.1mg
EUR 379

Goat/Sheep Anti-C. perfringens toxins (Alpha+beta+Epsilon) Antibody ELISA, 96 tests, Quantitative

RB-500730-1 100 ul
EUR 854.4

Cow/Bovine Anti-C. perfringens toxins (Alpha+beta+Epsilon) Antibody ELISA, 96 tests, Quantitative

RB-500740-1 100 ul
EUR 854.4

OASA00160-100UG - TCR Alpha/Beta Chain Antibody - FITC Conjugated

OASA00160-100UG 0.1mg
EUR 379

Mouse alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like (MGAT4D) Control/blocking peptide

AB-23037-P 100ug
EUR 196.8

T-Cell Receptor Alpha/Beta (TCR a/b) Antibody (PE / Cyanine 5)

abx347155-100g 100 µg Ask for price

T-Cell Receptor Alpha/Beta (TCR a/b) Antibody (PE / Cyanine 5)

abx347155-20g 20 µg
EUR 400

T-Cell Receptor Alpha/Beta (TCR a/b) Antibody (PE / Cyanine 5)

abx347155-50g 50 µg Ask for price

Rabbit Anti-Mouse alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like (MGAT4D) IgG (aff pure)

AB-23037-A 100ug
EUR 578.4

OASA00161-500UG - TCR Alpha Beta Beat Chain Antibody - FITC Conjugated

OASA00161-500UG 0.5mg
EUR 569

Human Anti-Parietal cell (alpha and beta subunits of the Parietal Cell (H//K/ATPase) IgG ELISA kit, 96 tests, Quantitative

3300-315-PRG 1 kit
EUR 708

T Cell Receptor (TCR) alpha/beta (alpha chain) mouse monoclonal antibody, clone 3A8, Purified

SM1230P 100 µg Ask for price

T Cell Receptor (TCR) alpha/beta (alpha chain) mouse monoclonal antibody, clone 3A8, Purified

SM1230PS 50 µg Ask for price

TCR alpha antibody

20R-1837 100 ug
EUR 807.6
Description: Rabbit polyclonal TCR alpha antibody

TCR alpha antibody

70R-12400 100 ug
EUR 392
Description: Rabbit polyclonal TCR alpha antibody

TCR alpha Antibody

5648-100 each
EUR 424.8

TCR alpha Antibody

5648-30T each
EUR 175.2

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone IP26, PE

AM26003RP-N 100 Tests Ask for price

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone IP26, APC

AM26003AC-N 100 Tests Ask for price

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone R73, FITC

SM321F 100 µg Ask for price

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone IP26, FITC

AM05684FC-N 100 Tests Ask for price

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone IP26, FITC

AM26003FC-N 100 Tests Ask for price

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone IP26, PerCP

AM26003PP-N 100 Tests Ask for price

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone IP26, Biotin

AM26003BT-N 100 µg Ask for price

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone R73, Purified

SM321P 250 µg Ask for price

Recombinant (E.Coli) Human p38 alpha/SAPK2 alpha

RP-675 1 ug
EUR 343.2

T Cell Receptor (TCR) alpha/beta hamster monoclonal antibody, clone H57-597, PE

CL075R 50 µg Ask for price

T Cell Receptor (TCR) alpha/beta hamster monoclonal antibody, clone H57-597, PE

CL075RX 300 µg Ask for price

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone IP26, Aff - Purified

AM26003PU-N 100 µg Ask for price

T Cell Receptor (TCR) alpha/beta hamster monoclonal antibody, clone H57-597, FITC

CL075F 100 µg Ask for price

T Cell Receptor (TCR) alpha/beta hamster monoclonal antibody, clone H57-597, FITC

CL075FX 300 µg Ask for price

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone R73, Low Endotoxin

SM321LE 500 µg Ask for price

T Cell Receptor (TCR) alpha/beta hamster monoclonal antibody, clone H57-597, Biotin

CL075B 100 µg Ask for price

T Cell Receptor (TCR) alpha/beta hamster monoclonal antibody, clone H57-597, Biotin

CL075BX 300 µg Ask for price

TRAC Antibody / TCR alpha

RQ4617 100ug
EUR 356.15
Description: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor alpha and delta loci. Both the alpha and delta loci include V (variable), J (joining), and C (constant) segments and the delta locus also includes diversity (D) segments. The delta locus is situated within the alpha locus, between the alpha V and J segments. During T cell development, the delta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The alpha chain is synthesized by recombination joining a single V segment with a J segment. For both chains, the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Five variable segments can be used in either alpha or delta chains and are described by TRAV/DV symbols. Several V and J segments of the alpha locus are known to be incapable of encoding a protein and are considered pseudogenes.

human Topoisomerase II alpha (TOP2 alpha) Control/blocking peptide

TOP2A11-P 100 ug
EUR 196.8

TCR Alpha Chain Antibody

GWB-805D28 0.1 mg Ask for price

TCR Alpha Chain Antibody

GWB-B1B1B6 0.05 mg Ask for price

Recombinant purified, human Interleukin-1 alpha (IL-1 alpha), active

IL1A15-R-10 10 ug
EUR 416.4

TCR alpha Blocking Peptide

33R-10580 50 ug
EUR 418.8
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of TCR alpha antibody, catalog no. 20R-1837

TCR alpha Blocking Peptide

33R-11053 50 ug
EUR 145
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of TCR alpha antibody, catalog no. 70R-12400

TCR alpha Blocking Peptide

5648BP-50 each
EUR 183.6

Rabbit Anti-human Topoisomerase II alpha (TOP2 alpha) IgG, aff pure

TOP2A11-A 100 ug
EUR 578.4

Polyclonal TCR alpha Antibody

AMM08788G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TCR alpha . This antibody is tested and proven to work in the following applications:

TCR beta antibody

10R-6585 100 ug
EUR 125
Description: Armenian Hamster monoclonal TCR beta antibody

TCR beta antibody

20R-1838 100 ug
EUR 807.6
Description: Rabbit polyclonal TCR beta antibody

He strongly inspired younger reproductive biologists who labored in his laboratory, and applauded the world-wide distinction of his scholar and affiliate, R. Yanagimachi, as a specialist in mammalian fertilisation. Finally, Chang’s continued emotions in the direction of his homeland are contrasted with the actuality of his American life after 1945, itself a examine in poignancy.

Study protocol: E-freeze - freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

Study protocol: E-freeze – freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

Infertility impacts one in seven {{couples}}; many of these need in vitro fertilisation (IVF). IVF entails exterior hormones to stimulate a girl’s ovaries to produce eggs which might be harvested surgically. Embryos, created in the laboratory by mixing eggs with sperm, are grown in custom for a few days sooner than being modified inside the uterus (fresh embryo transfer).

Spare embryos are sometimes frozen with a view to transfer at a later degree in time – significantly if the preliminary fresh transfer does not consequence in a being pregnant. Despite enhancements in know-how, IVF success costs keep low with an complete reside starting value of 25-30% per remedy. Additionally, there are issues about nicely being outcomes for mothers and infants conceived by IVF, notably after fresh embryo transfer, collectively with maternal ovarian hyperstimulation syndrome (OHSS) and preterm provide.

It is believed that prime ranges of hormones all through ovarian stimulation could create a comparatively hostile setting for embryo implantation whereas rising the menace of OHSS. It has been steered that freezing all embryos with the intention of thawing and altering them inside the uterus at a later stage (thawed frozen embryo transfer) as a substitute of fresh embryo transfer, would possibly outcome in improved being pregnant costs and fewer issues. We goal to examine the clinical and cost effectiveness of fresh and thawed frozen embryo transfer, with the main goal of determining any distinction in the likelihood of having a healthful baby.

E-Freeze is a pragmatic, multicentre two-arm parallel group randomised controlled trial the place women aged ≥18 and < 42 years, with not lower than three good prime quality embryos are randomly allotted to acquire each a fresh or thawed frozen embryo transfer.

The main remaining result’s a healthful baby, outlined as a time interval, singleton, reside starting with relevant weight for gestation. Cost effectiveness may be calculated from a healthcare and societal perspective.E-Freeze will determine the relative benefits of fresh and thawed frozen embryo transfer in phrases of enhancing the likelihood of having a healthful baby. The outcomes of this pragmatic study have the potential to be straight transferred to clinical observe.ISRCTN registry

 Study protocol: E-freeze - freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

Study protocol: E-freeze – freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

In vitro fertilisation/intracytoplasmic sperm injection previous 2020.

Over eight million infants have been born following IVF (in vitro fertilisation) and completely different artificial reproductive know-how (ART) procedures since Louise Brown’s starting 40 years in the previous. New enhancements have added a lot complexity to every clinical and laboratory procedures over the remaining four a few years. Translation of novel approaches from major science into clinical observe continues unabated, widening the applicability of ART to new groups of people and serving to boost every chances of healthful reside starting and affected particular person acceptability.

STAT Double Staining Reagent for Double staining o

NB327DB 25 ml
EUR 595
Description: STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 276

HE Staining Kit

E-IR-R117-100mL 100mL
EUR 240

HE Staining Kit

E-IR-R117-10mL 10mL
EUR 35

HE Staining Kit

E-IR-R117-50mL 50mL
EUR 150

HE Staining Kit

E-IR-R117-each each Ask for price

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

TRAP Staining Kit

KT-008 1 kit
EUR 747

DAPI Staining Kit

abx097206-100l 100 µl
EUR 162.5

DAPI Staining Kit

abx097206-1ml 1 ml Ask for price

DAPI Staining Kit

abx097206-200l 200 µl Ask for price

Coplin Staining Jar

08415-3 3sets
EUR 84

Silver Staining Kit

ML123-1KT 1 unit
EUR 201.16
Description: Silver Staining Kit

Cell Staining Buffer

E-CK-A107-100mL 100 mL
EUR 20

Cell Staining Buffer

E-CK-A107-200mL 200 mL
EUR 35

Cell Staining Buffer

E-CK-A107-500mL 500 mL
EUR 60

Cell Staining Buffer

E16FXP005 500 ml
EUR 40
Description: Available in various conjugation types.

DNA Staining Reagent

abx299713-100g 100 µg Ask for price

DNA Staining Reagent

abx299713-20g 20 µg
EUR 300

DNA Staining Reagent

abx299713-50g 50 µg Ask for price

Cell Staining Buffer

E-CK-A107-each each Ask for price

ELVIS ID Staining Kit

SK-ELVIS-100 100 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-1000 1000 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-200 200 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-500 500 Tests Ask for price

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 276

Hoechst 33342 Staining Kit

abx097207-100l 100 µl
EUR 162.5

Hoechst 33342 Staining Kit

abx097207-1ml 1 ml Ask for price

Hoechst 33342 Staining Kit

abx097207-200l 200 µl Ask for price

DNA STAINING AGENT (1ML)

NOVELJUICE each
EUR 80

Cell Cycle Staining Kit

KTA2020-100T 100 T
EUR 119
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-50T 50 T
EUR 69
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-each each Ask for price

Hematoxylin Staining Buffer

E-IR-R120-15mL 15mL
EUR 60

Hematoxylin Staining Buffer

E-IR-R120-50mL 50mL
EUR 170

Hematoxylin Staining Buffer

E-IR-R120-5mL 5mL
EUR 25

Hematoxylin Staining Buffer

E-IR-R120-each each Ask for price

Live NucRed staining kit

EGY069 >100T
EUR 210

PI / RNase Staining Buffer

20-abx090619
  • Ask for price
  • Ask for price
  • 100 tests
  • 50 tests

PI / RNase Staining Buffer

abx090619-100l 100 µl
EUR 162.5

PI / RNase Staining Buffer

abx090619-1ml 1 ml Ask for price

PI / RNase Staining Buffer

abx090619-200l 200 µl
EUR 200

HISTOCHEMISTRY STAINING TRAY

ATC-12 12 PLACE - CLEAR LID
EUR 226.1

HISTOCHEMISTRY STAINING TRAY

ATC-24 24 PLACE - CLEAR LID
EUR 342.21

HISTOCHEMISTRY STAINING TRAY

AT-12 12 PLACE - BLACK LID
EUR 226.1

HISTOCHEMISTRY STAINING TRAY

AT-24 24 PLACE - BLACK LID
EUR 342.21

Exosome fluorescent staining

S104 -
EUR 800

Stains All Gel Staining Kit

KT-792 20 tests
EUR 737

Live NucGreen staining kit

EGY068 >100T
EUR 210

Dead NucGreen staining kit

EGY070 >100T
EUR 210

GEL STAINING/INCUBATION BOX

SB-10 LIGHT SHIELDING
EUR 94.44

Ponceau S Staining Solution

41640009-1 500 mL
EUR 40.13

Ponceau S Staining Solution

41640009-2 1 L
EUR 58.16

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 58

Ponceau S Staining Solution

B7778-100000 100 g
EUR 78
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes

Ponceau S Staining Solution

B7778-50000 50 g
EUR 53
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes

Ponceau S Staining Solution

PW005 100ml
EUR 82.97

Ponceau S Staining Solution

EZWB08-1x-100mL 100mL
EUR 9.6

Ponceau S Staining Solution

EZWB08-1x-500mL 500mL
EUR 32.4

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 494.4

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 101.58

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 500
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 314.4

PI Viability Staining Solution

E16FXP002 150 tests
EUR 40
Description: Available in various conjugation types.

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 390.96

β-Galactosidase Staining Kit

AKR-100 1 kit
EUR 345

Methyl Green Staining Solution

abx090698-100l 100 µl
EUR 125

Methyl Green Staining Solution

abx090698-1ml 1 ml Ask for price

Methyl Green Staining Solution

abx090698-200l 200 µl
EUR 187.5

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 203

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 300
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 208.8

Live-Dead Cell Staining Kit

GWB-AXR262 100 stainings Ask for price

Live-Dead Cell Staining Kit

K501-100 each
EUR 561.6

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 552

Live-Dead Cell Staining Kit

K2081-1000 1000T
EUR 112

Live-Dead Cell Staining Kit

K2081-500 500T
EUR 68

EZ-Quick Slide Staining Set

IW-2510 -
EUR 425

Nerve Terminal Staining Kit I

70030-1 KT
EUR 605

Nerve Terminal Staining Kit V

70034 1SET
EUR 605
Description: N/A

Nerve Terminal Staining Kit V

70034-1 KT
EUR 605

Purple-Color AP Staining Kit

AP100P-1 100 Assays
EUR 235

Human Histochemistry Staining Kit

20-abx299039
  • Ask for price
  • Ask for price
  • Ask for price
  • 150 tests
  • 500 tests
  • 50 tests

Beta-Galactosidase Staining Kit

GWB-AXR372 200 assays. Ask for price

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 274

Beta-Galactosidase Staining Kit

K802-250 each
EUR 352.8

Slides Staining Jar, Schifferdecker

CG146-5X1NO 1 unit
EUR 53.59
Description: Slides Staining Jar, Schifferdecker

Lipid (Oil Red O) staining Kit

K580-24 each
EUR 542.4

Nerve Terminal Staining Kit IIA

70031-1 1SET
EUR 322
Description: N/A

Nerve Terminal Staining Kit IIB

70031-1-1 KT
EUR 395

Nerve Terminal Staining Kit III

70032-1 KT
EUR 513

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 418
Description: Detects activated Caspases by FACS, FL or plate reader

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 285.6

Manual Staining System (12 Wells)

IMS001 1 ea.
EUR 246.09

Ponceau S Staining Solution, 10×

EZWB08-10x-10mL 10mL
EUR 9

Ponceau S Staining Solution, 10×

EZWB08-10x-50mL 50mL
EUR 28.8

DNA Ploidy Analysis Staining Kit

K1439-1 each
EUR 705.6

DNA Ploidy Analysis Staining Kit

K1439-5 each
EUR 2402.4

Prime Juice DNA Staining Reagent

LD011-1000 1 ml
EUR 10

Novel Green DNA Staining Reagent

abx299721-100g 100 µg Ask for price

Novel Green DNA Staining Reagent

abx299721-20g 20 µg
EUR 325

Novel Green DNA Staining Reagent

abx299721-50g 50 µg Ask for price

Propidium Iodide (PI) Staining Kit

abx097208-100l 100 µl
EUR 162.5

Propidium Iodide (PI) Staining Kit

abx097208-1ml 1 ml Ask for price

Propidium Iodide (PI) Staining Kit

abx097208-200l 200 µl Ask for price

DAPI staining kit (ready to use) 

EGY0621 10mL
EUR 85

DAPI staining kit (ready to use) 

EGY0622 50mL
EUR 185

Alcian Blue (pH 2.5) Staining Kit

AFR-1 250 ml ea.
EUR 127.28

Alcian Blue (pH 2.5) Staining Kit

AFR-2 30 ml ea.
EUR 63

7-AAD Viability Staining Buffer

abx090617-100tests 100 tests
EUR 260.4

Plastic Coplin Staining Jars, each

IW-2501 -
EUR 8.6

7-AAD Viability Staining Buffer

abx090617-100l 100 µl
EUR 150

7-AAD Viability Staining Buffer

abx090617-1ml 1 ml Ask for price

7-AAD Viability Staining Buffer

abx090617-200l 200 µl Ask for price

Stayrightâ„¢ Purple HRP Staining Kit

45905 5 mL
EUR 112
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45905-5mL 5 mL
EUR 109
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45906 50 mL
EUR 341
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45906-50mL 50 mL
EUR 334
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Hematoxylin and Eosin Staining Kit

K1142-1000 2×500mL
EUR 62
Description: Hematoxylin and Eosin Staining,one of the most widely used staining technique in histopathology

TRAP Staining Kit with Osteoplates

KT-651 1 kit
EUR 1003

Hochst33342/PI Double Staining Kit

E37F032-100-tests 100tests
EUR 66.67
Description: Double staining kit

PAP PEN FOR IMMUNO STAINING - EACH

Z377821-1EA EACH
EUR 192.41

7-AAD Viability Staining Solution

E16FXP003 150 tests
EUR 145
Description: Available in various conjugation types.

7-AAD Viability Staining Solution

EXB0026 400 tests
EUR 44

Nalgene Box Staining 13x13x5cm - EACH

BOX1040 EACH
EUR 54

SafePinky DNA Gel Staining Solution

S1000-050 500ml
EUR 157.2

SafePinky DNA Gel Staining Solution

S1000-100 2X500ml
EUR 225.6

SafePinky DNA Gel Staining Solution

S1000-200 4X500ml
EUR 336

Disposable Slide Staining Trays - PK4

MIC5070 PK4
EUR 101.25

Slides Staining Jar, Hellendhal Type

CG145-5X1NO 1 unit
EUR 61.59
Description: Slides Staining Jar, Hellendhal Type

Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
EUR 292.8

Wheaton staining dishes 3 EA - PK3

S5017-3EA PK3
EUR 152.55

Calcein AM/PI Double Staining Kit

E-CK-A354-each each Ask for price
Description: Viability/Cytotoxicity

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 613
Description: Caspase Staining Kit for use activity in the research laboratory

Staining Trough+Lid Rectangular - EACH

MIC6008 EACH
EUR 31.05

Ponceau S Protein Staining Solution

PS003-B500ML 500 ml
EUR 25

Mouse to Mouse HRP Staining System

MTM003 400 Slides
EUR 249.43

Slides Staining Jar, with Ground - in

CG147-5X1NO 1 unit
EUR 124
Description: Slides Staining Jar, with Ground - in

Preloading Fluorescent Staining Solution

abx460011-1096tests 10 × 96 tests Ask for price

Preloading Fluorescent Staining Solution

abx460011-596tests 5 × 96 tests Ask for price

Preloading Fluorescent Staining Solution

abx460011-96tests 96 tests
EUR 250

Staining Jar PP + Lid 10 Slide - EACH

MIC6002 EACH
EUR 75.6

Coomassie Blue Fast Staining Solution

AR0170 100mL(for 10 assays to stain gels of 5 x 8.5cm)
EUR 96

Alkaline Phosphatase Red Staining kit

TBS2080 50 tests
EUR 89

Coomassie Blue Fast Staining Solution

EZWB15-500mL 500mL
EUR 14.4

Calcein-AM/PI Double Staining Kit

E-CK-A354-100Assays 100 Assays
EUR 100

Calcein-AM/PI Double Staining Kit

E-CK-A354-2000Assays 2000 Assays
EUR 600

Calcein-AM/PI Double Staining Kit

E-CK-A354-500Assays 500 Assays
EUR 300

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 610
Description: Detects activated Caspases-3 by FACS, FL or plate reader

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 285.6

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 490
Description: Detects activated Caspases-8 by FACS, FL or plate reader

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 285.6

Red Active Caspase-9 Staining Kit

K2055-100 100 assays
EUR 610
Description: Detects activated Caspases-9 by FACS, FL or plate reader

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 285.6

Slides Staining Racks for 30 pieces,

CG149-5X1NO 1 unit
EUR 124
Description: Slides Staining Racks for 30 pieces,

Calcein-AM/PI Double Staining Kit

E37F030-500-tests 500tests
EUR 266.67
Description: Double staining kit

Calcein-AM/PI Double Staining Kit

E37F031-1000-tests 1000tests
EUR 526.67
Description: Double staining kit

Trypan Blue Staining Cell Assay Kit

APB0003-10 5ml
EUR 0.1

Alkaline Phosphatase Blue Staining kit

TBS2085 50 tests
EUR 89

OOSB00015-500ML - Cell Staining Buffer

OOSB00015-500ML 500mL
EUR 119

OOSB00016-250ML - Cell Staining Buffer

OOSB00016-250ML 250mL
EUR 69

OOSB00017-100ML - Cell Staining Buffer

OOSB00017-100ML 100mL
EUR 39

OOTA00782-500ML - Flow Staining Buffer

OOTA00782-500ML 500mL
EUR 79

Four color suit nuclear staining Kit

EGY071 100T
EUR 1190

EasyDip Slide Staining Jar - Blue, each

M900-12B -
EUR 16.98

EasyDip Slide Staining Jar - Pink, each

M900-12P -
EUR 16.98

Staining Jar W Lid 115x115x77mm - PK10

MIC6016 PK10
EUR 481.95

Hoechst 33342 staining kit (ready to use) 

EGY0631 10mL
EUR 50

Hoechst 33342 staining kit (ready to use) 

EGY0632 50mL
EUR 160

Hoechst 33258 staining kit (ready to use) 

EGY0641 10mL
EUR 50

Hoechst 33258 staining kit (ready to use) 

EGY0642 50mL
EUR 145

Foxp3/Transcription Factor Staining Kit

E-CK-A108-20Assays 20 Assays
EUR 75

Fluorescein Active Caspase Staining Kit

K2047-100 100 assays
EUR 610
Description: Detects activated Caspases by FACS, FL or plate reader

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 285.6

Foxp3/Transcription Factor Staining Kit

E-CK-A108-each each Ask for price

Yeast Viability Staining Kit (Red/Blue)

31063-2-1 KT
EUR 475

Human Type I Collagen Staining Other

9044 1 kit
EUR 233
Description: Tissue Slides, Cultured Cells

EasyDip Slide Staining Jar - Green, each

M900-12G -
EUR 16.98

EasyDip Slide Staining Jar - White, each

M900-12W -
EUR 16.98

However, the have an effect on of ART on the nicely being of every victims and their offspring continues to set off concern, and many ethical challenges created by new scientific developments in this space entice extensively differing opinions.

What is apparent is that there may be a sustained world growth in utilisation of ART and that reproductive tourism will allow many people to entry the remedy they want nevertheless nationwide legal guidelines that may forbid some approaches. The greatest drawback is to broaden entry to ART to those residing in the a lot much less wealthy nations who’re equally deserving of its benefits.