Compare rec. lab reagents for research




Suppliers for Lab polyclonals

Phycocyanin dye

85R-1016 Fitzgerald 1 mg
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies

Phycoerythrin dye

85R-1018 Fitzgerald 1 mg
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies

Dye removal bag

DD4905007 Scientific Laboratory Supplies EACH

DNA Loading Dye

G030 ABM 6X; 3 x 1.0 ml

Dye Solution A

IRDSA01 Scientific Laboratory Supplies 100ML

Green-DNA Dye

SB2040 Bio Basic 100UL, 100UL

PCR SuperMix (+dye)

20-abx098003 Abbexa
  • 15 ml
  • 1 ml
  • 480 ml
  • 5 ml

rec EGF (human)

H-7490.0100 Bachem 0.1mg 194.4 EUR

rec EGF (human)

H-7490.0500 Bachem 0.5mg 457.2 EUR

pGADT7- Rec- 53

PVT11254 Lifescience Market 2 ug 444 EUR

rec Leptin (human)

H-5578.0200 Bachem 0.2mg 194.4 EUR

rec Leptin (human)

H-5578.1000 Bachem 1.0mg 457.2 EUR

rec Leptin (mouse)

H-5582.0200 Bachem 0.2mg 194.4 EUR

rec Leptin (mouse)

H-5582.1000 Bachem 1.0mg 457.2 EUR

pGADT7- Rec Plasmid

PVT4025 Lifescience Market 2 ug 390 EUR

Our used References in Pubmed.

rec GM-CSF (murine)

H-7380.0010 Bachem 10.0µg 457.2 EUR

rec GM-CSF (murine)

H-7380.0020 Bachem 20.0µg 868.8 EUR

rec FGF basic (human)

H-7535.0010 Bachem 10.0µg 194.4 EUR

rec FGF basic (human)

H-7535.0050 Bachem 50.0µg 457.2 EUR

CCK-A Rec Antibody

abx022548-01ml Abbexa 0.1 ml 1144.8 EUR

Compare monoclonal lab reagents for research



Suppliers for Lab recombinants

Urocortin, rat

HY-P1296 MedChemExpress 5mg

Pneumadin (rat) )

SP-100080-1 Alpha Diagnostics 1 mg

Calcitonin, rat

5-00903 CHI Scientific 4 x 1mg

Intermedin (rat)

5-01405 CHI Scientific 4 x 1mg

Calcitonin (rat)

H-3072.0001 Bachem 1.0mg
Description: Sum Formula: C148H228N40O46S3; CAS# [11118-25-5]

Calcitonin (rat)

H-3072.0005 Bachem 5.0mg
Description: Sum Formula: C148H228N40O46S3; CAS# [11118-25-5]

Intermedin (rat)

H-6068.0500 Bachem 0.5mg
Description: Sum Formula: C226H361N75O64S2; CAS# [1816940-00-7] net

Monoclonal GR monoclonal antibody

AMM00029G Leading Biology 0.05mg 633.6 EUR

Monoclonal TBP monoclonal antibody

APR13720G Leading Biology 0.1ml 633.6 EUR

Monoclonal Rsf1 monoclonal antibody

AMM07673G Leading Biology 0.05mg 633.6 EUR

Monoclonal Rsf1 monoclonal antibody

AMM07674G Leading Biology 0.1ml 633.6 EUR

Monoclonal EZH2 monoclonal antibody

AMM00030G Leading Biology 0.05mg 633.6 EUR

Monoclonal SirT1 monoclonal antibody

APR09951G Leading Biology 0.05mg 580.8 EUR

Monoclonal SirT1 monoclonal antibody

APR09952G Leading Biology 0.1ml 580.8 EUR

Monoclonal HDAC2 monoclonal antibody

AMM00031G Leading Biology 0.05mg 633.6 EUR

Our used antibodies in Pubmed.

pH 9-14 Test Strips

GR238014 Genorise Scientific Pack of 100 10 EUR

pH 1-14 Test Strips

GR238015 Genorise Scientific Pack of 100 10 EUR

PRL (Prolactin) ELISA test

4 Biobase 96T/Box Ask for price

FERR ( Ferritin) ELISA test

7 Biobase 96T/Box Ask for price

Urinary Indican Test Kit

I1000N BioAssay Systems 20 299 EUR

recombinant Lab Reagents for Research





Promoted Lab polyclonals

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-20tests Accu test 20 tests 283.2 EUR

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-40tests Accu test 40 tests 385.2 EUR

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)

GEN-402-25tests All test 25 tests 292.8 EUR

Test product

testttt National Diagnostics 0 Ask for price

Human TES(Testin) ELISA Kit

EH1738 FN Test 96T 681.12 EUR

T(Testosterone) ELISA Kit

EU0400 FN Test 96T 571.5 EUR

REN ELISA Kit| Rat Renin ELISA Kit

EF017245 Lifescience Market 96 Tests 826.8 EUR

Pir ELISA Kit| Rat Pirin ELISA Kit

EF019186 Lifescience Market 96 Tests 826.8 EUR

Kl ELISA Kit| Rat Klotho ELISA Kit

EF017474 Lifescience Market 96 Tests 826.8 EUR

LN ELISA Kit| Rat Laminin ELISA Kit

EF017041 Lifescience Market 96 Tests 826.8 EUR

Lep ELISA Kit| Rat Leptin ELISA Kit

EF017074 Lifescience Market 96 Tests 826.8 EUR

GT ELISA Kit| Rat Gastrin ELISA Kit

EF017314 Lifescience Market 96 Tests 826.8 EUR

Mefv ELISA Kit| Rat Pyrin ELISA Kit

EF018946 Lifescience Market 96 Tests 826.8 EUR

Our used rec. in Pubmed.

Anti-Anti-SEPT1 antibody antibody

STJ119580 St John's Laboratory 100 µl 332.4 EUR

Anti-Anti-SEPT6 antibody antibody

STJ11100949 St John's Laboratory 100 µl 332.4 EUR

Anti-Anti-SEPT9 Antibody antibody

STJ111369 St John's Laboratory 100 µl 332.4 EUR

Anti-Anti-SEPT4 Antibody antibody

STJ112276 St John's Laboratory 100 µl 332.4 EUR

Anti-Anti-SEPT7 Antibody antibody

STJ116214 St John's Laboratory 100 µl 332.4 EUR

Anti-Anti-SEPT8 Antibody antibody

STJ117206 St John's Laboratory 100 µl 332.4 EUR

Anti-Anti-SEPT2 Antibody antibody

STJ25475 St John's Laboratory 100 µl 332.4 EUR

Anti-Anti-SEPT5 Antibody antibody

STJ25477 St John's Laboratory 100 µl 332.4 EUR

Anti-Anti-SEPT8 Antibody antibody

STJ25479 St John's Laboratory 100 µl 332.4 EUR

Ly1 Antibody Reactive (LYAR) Antibody

20-abx311665 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Focus Simplexa NATtrol

They represent the preferred industry standard for molecular diagnostic testing and can be used as independent (third party) quality control materials. NATtrol products are prepared from purified microorganisms that are grown in cell culture, in microbial culture, or isolated from the plasma of infected individuals.

NATtrol treatment modifies surface proteins and makes organisms non-infectious and stable in the refrigerator while retaining the complete genome. Inactivation of the organisms is verified by the absence of growth invalidated infectivity tests based on tissue cultures or in validated growth protocols (as appropriate)

  • Non-infectious and stable in the refrigerator
  • Purified and intact organisms
  • Comprehensive process controls to monitor extraction and amplification steps
  • Available in different formats (one or more analytes)
  • Can be used on various molecular assay platforms and analyzes
  • Traceable to WHO international standards (if applicable)
  • Shelf life of 12 to 24 months
  • Extensive coverage of patents * and/or pending patents

Frequent use of NATtrol controls can be used to:

  • Train and supervise laboratory staff
  • Assess lot-to-lot consistency of test kits and test reagents
  • Monitor daily variations in reported results
  • Provide an impartial and independent assessment of the competence of the tests
  • Provide consistent, reliable, and accurate quality control solutions
  • Help identify lab trends

3 COMMON PROBLEMS WHEN DOING AN ELISA

The ELISA immunoassays are among the most versatile to detect, identify and / or quantitate a particular antigen present in our sample. It is a highly sensitive, robust and relatively inexpensive test, quick and easy to carry out.

But despite the apparent simplicity of its protocol, as with most biological assays, there are times when the results are not as expected.

In this post we will focus on  3 common problems when doing an ELISA , and we will try to give some guidelines to be able to  solve them successfully .

Some of the common problems when doing an ELISA are obtaining a weak , low intensity or even non-existent signal , generating a high background noise that distorts the results, or the high inconsistency of the results between wells .

Next we leave you a list of Possible Causes (PC) and Solutions (S) for these 3 common problems when doing an ELISA.

1.- THE SIGNAL INTENSITY IS VERY LOW OR NON-EXISTENT:

PC: Problems with the protocol

S: The essay may not have been prepared correctly, and to fix it it is necessary to review several points:

  • Check that each step of the protocol has been applied correctly
  • Check that the wavelength and filters used in the plate reader are appropriate. For this, the test can be repeated using a positive control.

PC: Antibody problems

S: Antibodies are the most critical reagents in ELISA tests, and it is important to pay attention to the following points:

  • Use the dilution recommended by the manufacturer, or failing that, optimize the dilution of the antibody to be used.
  • Make sure you use enough antibodies, testing different concentrations of the antibodies to identify the optimal concentration for your assay.
  • Make sure that the antibodies have been stored correctly and have not undergone excessive freeze / thaw cycles .

PC: Problems with antigen

S: When the plates are covered with the antigen and when adding the primary antibody the signal is weak or null, there are two main aspects to check:

  • Sometimes it is possible that a sufficient amount of antigen has not adhered to the plate, try adding more.
  • In the case of peptide antigens, they must be previously conjugated to a large carrier protein in order to facilitate the recognition of the epitope by the antibody.

PC: Problems with reagents

S: Always make sure that:

  • The reagents are at room temperature before starting the assay
  • Check expiration dates
  • Check that they have been prepared correctly and have been added in the corresponding order.
  • Make sure that they do not interfere with other buffers or compounds present in the sample such as sodium acid, EDTA, etc.
  • Avoid mixing reagents from different kits.

PC: Low incubation times and temperature

S: Be sure to incubate the samples for the time specified by the manufacturer and optimize the incubation temperature for your assay. Remember that the reagents must be at room temperature before starting the assay.

PC: Storage conditions

S: Stores reagents to your specifications, keeping in mind that all reagents may not need the same storage requirements.

2.- THE BACKGROUND NOISE IS VERY HIGH

PC: Antibody problems

S: Regarding antibodies, there can be several cases:

  • That the concentration of antibody used is too high. This will be resolved by reducing its concentration, or by carrying out an optimization test to determine the ideal concentration.
  • There are cross reactivity problems, for which a negative control should be used.
  • Non-specific binding where the antibody is detecting other molecules besides the target antigen. The solution is through the use of affinity purified antibodies and the use of the appropriate blocking buffers .

PC: Problems with reagents

S: Regarding the different reagents, we must make sure that:

  • The stop solution is added to avoid an over development of the reaction.
  • The detection reagent has been properly diluted, and if so, retest with an even higher dilution.
  • The appropriate blocking buffers have been used and the blocking time has been sufficient.

PC: Incubation problems

S: In case of obtaining high background noise, it may be necessary to reduce the incubation temperature. On the other hand, it is necessary to ensure that the incubation of the substrate is carried out in dark conditions.

PC: Insufficient washes

S: Carefully wash the bottom of the plate and repeat the reading.

3.- THE VARIATION OF RESULTS BETWEEN WELLS IS VERY HIGH

PC: Inadequate incubation

S: Regarding the incubation step, it is important:

  • Do not stack the plates during the process to ensure that the temperature is evenly distributed.
  • Make sure there are no bubbles inside the wells before incubation.

PC: improper washing

S: Make sure to thoroughly wash all wells, as inhomogeneous washing could result in a high coefficient of variation.

 

 

How To Improve Protein Quality

When working with protein samples , there are critical factors that must be closely monitored to avoid their degradation or dysfunction and guarantee the reliability of the results.

These factors therefore reflect the quality of the proteins and can be basically summarized as:

  • Integrity
  • Purity
  • Homogeneity
  • Solubility
  • Stability
  • Storage

In this post we bring you some tips to improve the quality of proteins and thus optimize the results of the tests.

1.- OPTIMIZATION OF PROTEIN PURITY AND INTEGRITY

Optimizing the purity and integrity of proteins involves avoiding or minimizing contamination of the sample with impurities, modified proteins or degradation products, among others.

Some of the steps that can be taken to this end are:

  1. Change the purification protocol by modifying for example the washing and elution conditions or adding additional purification steps such as ion exchange chromatography.
  2. Modify the conditions of induction of protein expression
  3. Use a different cloning vector
  4. Use a different expression system

2.- OPTIMIZATION OF PROTEIN HOMOGENEITY AND SOLUBILITY

To prevent or eliminate the formation of aggregates and increase the solubility of the protein s interest, there are several points that can affect, such as:

  1. Always perform size exclusion chromatography as the last step in the protein purification process.
  2. Do not over-concentrate the sample, since the processes applied for this purpose usually induce the aggregation of proteins (Remember this entry about 5 techniques to concentrate proteins ).
  3. Modify the composition of the buffer in which the protein is located until optimizing the pH, salinity, additives, etc. best suited in each case.

3.- OPTIMIZATION OF THE STABILITY AND STORAGE OF PROTEINS

Regarding the stability and medium or long-term storage of proteins, it is common to think that freezing and cryopreservation is the ideal method. And although this is true in a large part of the cases, it is not always the case due to the intrinsic variability of each individual protein.

That is why it is advisable to study the stability of each protein of interest to identify the optimal storage method in each case, whether it be simple refrigeration, freezing, lyophilization, etc.

You can expand the information in this post on How to store proteins to avoid their degradation .