Study protocol: E-freeze – freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

Infertility impacts one in seven {{couples}}; many of these need in vitro fertilisation (IVF). IVF entails exterior hormones to stimulate a girl’s ovaries to produce eggs which might be harvested surgically. Embryos, created in the laboratory by mixing eggs with sperm, are grown in custom for a few days sooner than being modified inside the uterus (fresh embryo transfer).

Spare embryos are sometimes frozen with a view to transfer at a later degree in time – significantly if the preliminary fresh transfer does not consequence in a being pregnant. Despite enhancements in know-how, IVF success costs keep low with an complete reside starting value of 25-30% per remedy. Additionally, there are issues about nicely being outcomes for mothers and infants conceived by IVF, notably after fresh embryo transfer, collectively with maternal ovarian hyperstimulation syndrome (OHSS) and preterm provide.

It is believed that prime ranges of hormones all through ovarian stimulation could create a comparatively hostile setting for embryo implantation whereas rising the menace of OHSS. It has been steered that freezing all embryos with the intention of thawing and altering them inside the uterus at a later stage (thawed frozen embryo transfer) as a substitute of fresh embryo transfer, would possibly outcome in improved being pregnant costs and fewer issues. We goal to examine the clinical and cost effectiveness of fresh and thawed frozen embryo transfer, with the main goal of determining any distinction in the likelihood of having a healthful baby.

E-Freeze is a pragmatic, multicentre two-arm parallel group randomised controlled trial the place women aged ≥18 and < 42 years, with not lower than three good prime quality embryos are randomly allotted to acquire each a fresh or thawed frozen embryo transfer.

The main remaining result’s a healthful baby, outlined as a time interval, singleton, reside starting with relevant weight for gestation. Cost effectiveness may be calculated from a healthcare and societal perspective.E-Freeze will determine the relative benefits of fresh and thawed frozen embryo transfer in phrases of enhancing the likelihood of having a healthful baby. The outcomes of this pragmatic study have the potential to be straight transferred to clinical observe.ISRCTN registry

 Study protocol: E-freeze - freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

Study protocol: E-freeze – freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

In vitro fertilisation/intracytoplasmic sperm injection previous 2020.

Over eight million infants have been born following IVF (in vitro fertilisation) and completely different artificial reproductive know-how (ART) procedures since Louise Brown’s starting 40 years in the previous. New enhancements have added a lot complexity to every clinical and laboratory procedures over the remaining four a few years. Translation of novel approaches from major science into clinical observe continues unabated, widening the applicability of ART to new groups of people and serving to boost every chances of healthful reside starting and affected particular person acceptability.

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 230

ALP Antibody

DF12525 200ul
EUR 304
Description: ALP Antibody detects endogenous levels of ALP.

anti-ALP

YF-PA18460 50 ug
EUR 363
Description: Mouse polyclonal to ALP

anti-ALP

YF-PA18461 50 ul
EUR 363
Description: Mouse polyclonal to ALP

ALP-B/ Rat ALP- B ELISA Kit

ELA-E0473r 96 Tests
EUR 886

Ponceau S Staining Solution

PW005 100ml
EUR 69.14

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 174

PI / RNase Staining Buffer

20-abx090619
  • EUR 272.00
  • EUR 230.00
  • 100 tests
  • 50 tests

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 230

Human Histochemistry Staining Kit

20-abx299039
  • EUR 871.00
  • EUR 1219.00
  • EUR 565.00
  • 150 tests
  • 500 tests
  • 50 tests

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 325.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 293

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 176

Ponceau S Staining Solution

B7778-100000 100 g
EUR 163

Ponceau S Staining Solution

B7778-50000 50 g
EUR 126

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 370
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 58

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 379

Beta-Galactosidase Staining Kit

K802-250
EUR 294

Streptavidin Protein (ALP)

abx061009-2ml 2 ml
EUR 704

ALP Blocking Peptide

20-abx161265
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

ALP Blocking Peptide

DF12525-BP 1mg
EUR 195

7-AAD Viability Staining Solution

EXB0026 400 tests
EUR 76

SafePinky DNA Gel Staining Solution

S1000-050 500ml
EUR 131

SafePinky DNA Gel Staining Solution

S1000-100 2X500ml
EUR 188

SafePinky DNA Gel Staining Solution

S1000-200 4X500ml
EUR 280

Nerve Terminal Staining Kit V

70034 1SET
EUR 509
Description: Minimum order quantity: 1 unit of 1SET

Coomassie Blue Staining Destaining Solution

AR0140 1kit (for 10 assays to stain gels of 5 x 8.5cm)
EUR 70

Coomassie Blue Fast Staining Solution

AR0170 100mL(for 10 assays to stain gels of 5 x 8.5cm)
EUR 80

7-AAD Viability Staining Buffer

abx090617-100tests 100 tests
EUR 217

Manual Staining System (12 Wells)

IMS001 1 ea.
EUR 356

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 238

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 572

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 238

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 460

Stain Tray Slide Staining System

M920-1 1 Clear
EUR 428

Stain Tray Slide Staining System

M920-2 1 Black
EUR 445

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 262

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 412

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 262

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 638
Description: Live/Dead Cell Staining Kit for use in the research laboratory

DNA Ploidy Analysis Staining Kit

K1439-1
EUR 588

DNA Ploidy Analysis Staining Kit

K1439-5
EUR 2002

Live-Dead Cell Staining Kit

K501-100
EUR 468

Recombinant Alkaline Phosphatase (ALP)

4-RPB472Bo01
  • EUR 503.20
  • EUR 238.00
  • EUR 1612.00
  • EUR 604.00
  • EUR 1108.00
  • EUR 400.00
  • EUR 3880.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Bovine Alkaline Phosphatase expressed in: E.coli

Sheep ALP ELISA Kit

ESA0038 96Tests
EUR 521

Mouse ALP ELISA Kit

EMA0038 96Tests
EUR 521

Anserini ALP ELISA Kit

EAA0038 96Tests
EUR 521

Rabbit ALP ELISA Kit

ERTA0038 96Tests
EUR 521

Monkey ALP ELISA Kit

EMKA0038 96Tests
EUR 521

Porcine ALP ELISA Kit

EPA0038 96Tests
EUR 521

Rat ALP ELISA Kit

ERA0038 96Tests
EUR 521

Human ALP ELISA Kit

EHA0038 96Tests
EUR 521

Canine ALP ELISA Kit

ECA0038 96Tests
EUR 521

Chicken ALP ELISA Kit

ECKA0038 96Tests
EUR 521

Goat ALP ELISA Kit

EGTA0038 96Tests
EUR 521

Bovine ALP ELISA Kit

EBA0038 96Tests
EUR 521

Alkaline Phosphatase (ALP) Antibody

20-abx121265
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Endoplasmin (GRP94) Antibody (ALP)

abx447036-100ug 100 ug
EUR 537

Piccolo (Pclo) Antibody (ALP)

abx447495-100ug 100 ug
EUR 578

ubiquitin (Ubiquitin) Antibody (ALP)

abx447954-200ul 200 ul
EUR 620

Alkaline Phosphatase (ALP) Antibody

abx038456-100ug 100 ug
EUR 391

Protein A Antibody (ALP)

abx023329-2ml 2 ml
EUR 843

Endoplasmin (GRP94) Antibody (ALP)

abx442250-200ug 200 ug
EUR 578

GRP75 (Grp75) Antibody (ALP)

abx442270-100ug 100 ug
EUR 565

ubiquitin (Ubiquitin) Antibody (ALP)

abx442291-200ug 200 ug
EUR 620

ubiquitin (Ubiquitin) Antibody (ALP)

abx442300-200ug 200 ug
EUR 620

Rhodopsin (RHO) Antibody (ALP)

abx442303-100ug 100 ug
EUR 578

Rhodopsin (RHO) Antibody (ALP)

abx442304-100ug 100 ug
EUR 578

Synaptophysin (SYP) Antibody (ALP)

abx442305-100ug 100 ug
EUR 509

Syntaxin (STX) Antibody (ALP)

abx442308-100ug 100 ug
EUR 537

Piccolo (Pclo) Antibody (ALP)

abx442314-100ug 100 ug
EUR 565

Kir2.1 (Kir2.1) Antibody (ALP)

abx442362-100ug 100 ug
EUR 578

Kir2.2 (Kir2.2) Antibody (ALP)

abx442363-100ug 100 ug
EUR 578

VGLUT1 (VGLUT1) Antibody (ALP)

abx442402-100ug 100 ug
EUR 578

VGLUT2 (VGLUT2) Antibody (ALP)

abx442403-100ug 100 ug
EUR 578

Versican (VCAN) Antibody (ALP)

abx442447-100ug 100 ug
EUR 578

Malin (NHLRC1) Antibody (ALP)

abx442452-100ug 100 ug
EUR 578

Laforin (EPM2A) Antibody (ALP)

abx442472-100ug 100 ug
EUR 578

Kir6.1 (Kir6.1) Antibody (ALP)

abx442497-100ug 100 ug
EUR 578

Citrulline (CIT) Antibody (ALP)

abx442502-100ug 100 ug
EUR 662

Citrulline (CIT) Antibody (ALP)

abx442503-100ug 100 ug
EUR 662

ubiquitin (Ubiquitin) Antibody (ALP)

abx444810-100ug 100 ug
EUR 398

Calreticulin (CALR) Antibody (ALP)

abx445319-200ul 200 ul
EUR 551

Bassoon (BSN) Antibody (ALP)

abx446594-100ug 100 ug
EUR 578

Rubicon (Rubicon) Antibody (ALP)

abx446730-100ug 100 ug
EUR 578

Alkaline Phosphatase (ALP) Antibody

20-abx175316
  • EUR 1177.00
  • EUR 578.00
  • 1 mg
  • 200 ug

Alkaline Phosphatase (ALP) Antibody

20-abx175317
  • EUR 1247.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Metallothionein (mymT) Antibody (ALP)

abx448218-100ug 100 ug
EUR 578

Anti-ASRGL1 / ALP antibody

STJ71356 100 µg
EUR 359

ALP ELISA KIT|Human

EF009206 96 Tests
EUR 689

QuantiFluo ALP Detection Reagent

QFALP-6mL 600
EUR 156
Description: A stable 4-methylumbelliferyl phosphate (MUP) liquid substrate reagent formulated for detecting alkaline phosphatase ALP, e.g. in ELISA. This single reagent produces a highly fluorescent product (360/450 nm) when dephosphorylated by ALP. The fluorescent p

STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides

NB330DB 25 ml
EUR 740

Basic Fuchsin, for flagella staining, certified

GT2106-25G 25 g
EUR 229

Human Type I Collagen Staining Kit

9044 1 kit
EUR 338.55
Description: Human Type I Collagen Staining Kit

Yeast Viability Staining Kit (Green/Red)

31063-1 1kit
EUR 472
Description: Minimum order quantity: 1 unit of 1kit

StemTAG Alkaline Phosphatase Staining Kit (Red)

CBA-300 100 assays
EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

StemTAG Alkaline Phosphatase Staining Kit (Purple)

CBA-306 100 assays
EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Coomassie Brilliant Blue Fast Staining Solution

abx090652-200ml 200 ml
EUR 203

Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
EUR 189

Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
EUR 244

Human FoxP3 Transcription Factor Staining Kit

OKTA00001 KIT
EUR 454
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Human FoxP3 Transcription Factor Staining Kit

OKTA00004 KIT
EUR 454
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Mouse to Mouse HRP Staining System

MTM003 400 Slides
EUR 361

Fluorescein Active Caspase-12 Staining Kit

K2046-25 25 assays
EUR 238

Fluorescein Active Caspase-2 Staining Kit

K2048-25 25 assays
EUR 238

Fluorescein Active Caspase-3 Staining Kit

K2049-25 25 assays
EUR 238

Fluorescein Active Caspase-8 Staining Kit

K2050-25 25 assays
EUR 238

Fluorescein Active Caspase-9 Staining Kit

K2051-100 100 assays
EUR 572

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 238

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 572

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 238

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 572

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 238

Red Active Caspase-9 Staining Kit

K2055-100 100 assays
EUR 572

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 238

Vertical Slide Staining Rack Dark Gray

M905-12DGY 1 Case(s)
EUR 184

SensiTek HRP Anti-Mouse Staining System

AFA600 500 Slides
EUR 215

SensiTek HRP Anti-Rabbit Staining System

AFE600 500 Slides
EUR 215

SensiTek HRP Anti-Polyvalent Staining System

AFG600 500 Slides
EUR 370

UltraTek HRP Anti-Mouse Staining System

AFJ600 500 Slides
EUR 284

UltraTek HRP Anti-Rabbit Staining System

AFK600 500 Slides
EUR 284

UltraTek HRP Anti-Polyvalent Staining System

AFN600 500 Slides
EUR 284

Alcian Blue (pH 2.5) Staining Kit

AFR-1 250 ml ea.
EUR 208

Alcian Blue (pH 2.5) Staining Kit

AFR-2 30 ml ea.
EUR 128

CaspGLOW Active Caspase 12 Staining Kit

55R-1291 25 assays
EUR 282
Description: Caspase 12 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 2 Staining Kit

55R-1293 25 assays
EUR 284
Description: Caspase 2 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 3 Staining Kit

55R-1294 25 assays
EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 8 Staining Kit

55R-1295 25 assays
EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 9 Staining Kit

55R-1296 25 assays
EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase Staining Kit

55R-1297 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

Genorise® Alkaline Phosphatase Staining Kit

GR107045 100 tests
EUR 289

Coomassie Blue G-250 Staining Solution

GR103035 1 L
EUR 95

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-100
EUR 550

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-25
EUR 234

CaspGLOW? Red Active Caspase Staining Kit

K190-100
EUR 523

CaspGLOW? Red Active Caspase Staining Kit

K190-25
EUR 240

Lipid (Oil Red O) staining Kit

K580-24
EUR 452

Gel-FAST? Gel Staining/Destaining Kit

K901-40
EUR 175

STAT Double Staining reagent for Double staining of Mouse and Rabbit antibodies, 450 plus slides

NB326DB 25 ml
EUR 740

STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides

NB327DB 25 ml
EUR 740

Mouse ALP-B ELISA Kit

EMA0794 96Tests
EUR 521

Anserini ALP-B ELISA Kit

EAA0794 96Tests
EUR 521

Rabbit ALP-B ELISA Kit

ERTA0794 96Tests
EUR 521

Porcine ALP-B ELISA Kit

EPA0794 96Tests
EUR 521

Rat ALP-B ELISA Kit

ERA0794 96Tests
EUR 521

Human ALP-B ELISA Kit

EHA0794 96Tests
EUR 521

Canine ALP-B ELISA Kit

ECA0794 96Tests
EUR 521

Guinea Pig ALP ELISA Kit

EGA0038 96Tests
EUR 521

Goat ALP-B ELISA Kit

EGTA0794 96Tests
EUR 521

Bovine ALP-B ELISA Kit

EBA0794 96Tests
EUR 521

Human Alkaline Phosphatase (ALP) Antibody

32928-05111 150 ug
EUR 261

Goat Anti-human IgG-ALP

STJ15100026 1 ml
EUR 350
Description: This ALP-conjugate enables detection of human IgG in enzyme immunoassays such as indirect ELISA and Western blot.

Goat Anti-mouse IgA-ALP

STJ15100027 1 ml
EUR 350
Description: This ALP-conjugate enables detection of mouse IgA in enzyme immunoassays such as indirect ELISA and Western blot.

Rabbit Anti-mouse IgM-ALP

STJ15100028 1 ml
EUR 350
Description: This ALP-conjugate enables detection of mouse IgM in enzyme immunoassays such as indirect ELISA and Western blot.

Goat Anti-human IgM-ALP

STJ15100029 1 ml
EUR 350
Description: This ALP-conjugate enables detection of human IgM in enzyme immunoassays such as indirect ELISA and Western blot.

Goat Anti-human IgA-ALP

STJ15100030 1 ml
EUR 350
Description: This ALP-conjugate enables detection of human IgA in enzyme immunoassays such as indirect ELISA and Western blot.

Goat Anti-mouse IgG-ALP

STJ15100031 1 ml
EUR 350
Description: This ALP-conjugate enables detection of mouse IgG in enzyme immunoassays such as indirect ELISA and Western blot.

Goat Anti-rat IgG-ALP

STJ15100033 1 ml
EUR 350
Description: Goat anti-rat IgG-ALP is specific for rat IgG H-chains and was purified using immunoaffinity chromatography.

Goat Anti-rabbit IgG-ALP

STJ15100034 1 ml
EUR 350
Description: Goat anti-rabbit IgG-ALP is specific for rabbit IgG H- and L-chains and was purified using immunoaffinity chromatography.

Alkaline Phosphatase(ALP/597) Antibody

BNC040597-100 100uL
EUR 199
Description: Primary antibody against Alkaline Phosphatase(ALP/597), CF405S conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(ALP/597) Antibody

BNC040597-500 500uL
EUR 544
Description: Primary antibody against Alkaline Phosphatase(ALP/597), CF405S conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase (ALP) Antibody Pair

20-abx370189
  • EUR 1261.00
  • EUR 829.00
  • 10 × 96 tests
  • 5 × 96 tests

Cow Alkaline Phosphatase (ALP) Protein

abx670005-5mg 5 mg
EUR 398

Human Phosphatase (ALP) CLIA Kit

20-abx496324
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

GTPase Kras (RAS) Antibody (ALP)

abx447019-100ug 100 ug
EUR 523

Aquaporin 1 (Aqp1) Antibody (ALP)

abx447308-100ug 100 ug
EUR 578

Aquaporin 4 (AQP4) Antibody (ALP)

abx447325-100ug 100 ug
EUR 578

Aquaporin 2 (Aqp2) Antibody (ALP)

abx447342-100ug 100 ug
EUR 578

Aquaporin 3 (Aqp3) Antibody (ALP)

abx447631-100ug 100 ug
EUR 578

Beclin 2 (Becn2) Antibody (ALP)

abx447937-100ug 100 ug
EUR 578

Calnexin-CT (CANX) Antibody (ALP)

abx448030-200ul 200 ul
EUR 662

Calnexin-CT (CANX) Antibody (ALP)

abx448031-200ug 200 ug
EUR 676

Calnexin-NT (CANX) Antibody (ALP)

abx448056-200ul 200 ul
EUR 662

Beclin 2 (Becn2) Antibody (ALP)

abx448081-100ug 100 ug
EUR 578

Beclin 2 (Becn2) Antibody (ALP)

abx448082-100ug 100 ug
EUR 578

Human Alkaline Phosphatase (ALP) Enzyme

abx077002-100U 100 U
EUR 565

Alkaline Phosphatase(ALP/597) Antibody

BNC470597-100 100uL
EUR 199
Description: Primary antibody against Alkaline Phosphatase(ALP/597), CF647 conjugate, Concentration: 0.1mg/mL

Alkaline Phosphatase(ALP/597) Antibody

BNC470597-500 500uL
EUR 544
Description: Primary antibody against Alkaline Phosphatase(ALP/597), CF647 conjugate, Concentration: 0.1mg/mL

However, the have an effect on of ART on the nicely being of every victims and their offspring continues to set off concern, and many ethical challenges created by new scientific developments in this space entice extensively differing opinions.

What is apparent is that there may be a sustained world growth in utilisation of ART and that reproductive tourism will allow many people to entry the remedy they want nevertheless nationwide legal guidelines that may forbid some approaches. The greatest drawback is to broaden entry to ART to those residing in the a lot much less wealthy nations who’re equally deserving of its benefits.