Study protocol: E-freeze – freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

Infertility impacts one in seven {{couples}}; many of these need in vitro fertilisation (IVF). IVF entails exterior hormones to stimulate a girl’s ovaries to produce eggs which might be harvested surgically. Embryos, created in the laboratory by mixing eggs with sperm, are grown in custom for a few days sooner than being modified inside the uterus (fresh embryo transfer).

Spare embryos are sometimes frozen with a view to transfer at a later degree in time – significantly if the preliminary fresh transfer does not consequence in a being pregnant. Despite enhancements in know-how, IVF success costs keep low with an complete reside starting value of 25-30% per remedy. Additionally, there are issues about nicely being outcomes for mothers and infants conceived by IVF, notably after fresh embryo transfer, collectively with maternal ovarian hyperstimulation syndrome (OHSS) and preterm provide.

It is believed that prime ranges of hormones all through ovarian stimulation could create a comparatively hostile setting for embryo implantation whereas rising the menace of OHSS. It has been steered that freezing all embryos with the intention of thawing and altering them inside the uterus at a later stage (thawed frozen embryo transfer) as a substitute of fresh embryo transfer, would possibly outcome in improved being pregnant costs and fewer issues. We goal to examine the clinical and cost effectiveness of fresh and thawed frozen embryo transfer, with the main goal of determining any distinction in the likelihood of having a healthful baby.

E-Freeze is a pragmatic, multicentre two-arm parallel group randomised controlled trial the place women aged ≥18 and < 42 years, with not lower than three good prime quality embryos are randomly allotted to acquire each a fresh or thawed frozen embryo transfer.

The main remaining result’s a healthful baby, outlined as a time interval, singleton, reside starting with relevant weight for gestation. Cost effectiveness may be calculated from a healthcare and societal perspective.E-Freeze will determine the relative benefits of fresh and thawed frozen embryo transfer in phrases of enhancing the likelihood of having a healthful baby. The outcomes of this pragmatic study have the potential to be straight transferred to clinical observe.ISRCTN registry

 Study protocol: E-freeze - freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

Study protocol: E-freeze – freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

In vitro fertilisation/intracytoplasmic sperm injection previous 2020.

Over eight million infants have been born following IVF (in vitro fertilisation) and completely different artificial reproductive know-how (ART) procedures since Louise Brown’s starting 40 years in the previous. New enhancements have added a lot complexity to every clinical and laboratory procedures over the remaining four a few years. Translation of novel approaches from major science into clinical observe continues unabated, widening the applicability of ART to new groups of people and serving to boost every chances of healthful reside starting and affected particular person acceptability.

STAT Double Staining Reagent for Double staining o

NB327DB 25 ml
EUR 595
Description: STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 276

HE Staining Kit

E-IR-R117-100mL 100mL
EUR 240

HE Staining Kit

E-IR-R117-10mL 10mL
EUR 35

HE Staining Kit

E-IR-R117-50mL 50mL
EUR 150

HE Staining Kit

E-IR-R117-each each Ask for price

PI Staining Kit

MBS826722-01mL 0.1mL
EUR 140

PI Staining Kit

MBS826722-5x01mL 5x0.1mL
EUR 550

HE staining Kit

MBS2567140-100mL 100mL
EUR 255

HE staining Kit

MBS2567140-10mL 10mL
EUR 120

HE staining Kit

MBS2567140-50mL 50mL
EUR 195

HE staining Kit

MBS2567140-5x100mL 5x100mL
EUR 1120

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

TRAP Staining Kit

KT-008 1 kit
EUR 747

DAPI Staining Kit

abx097206-100l 100 µl
EUR 162.5

DAPI Staining Kit

abx097206-1ml 1 ml Ask for price

DAPI Staining Kit

abx097206-200l 200 µl Ask for price

DAPI Staining Kit

MBS826728-01mL 0.1mL
EUR 140

DAPI Staining Kit

MBS826728-5x01mL 5x0.1mL
EUR 550

Coplin Staining Jar

08415-3 3sets
EUR 84

Silver Staining Kit

ML123-1KT 1 unit
EUR 201.16
Description: Silver Staining Kit

Cell Staining Buffer

E-CK-A107-100mL 100 mL
EUR 20

Cell Staining Buffer

E-CK-A107-200mL 200 mL
EUR 35

Cell Staining Buffer

E-CK-A107-500mL 500 mL
EUR 60

Cell Staining Buffer

E16FXP005 500 ml
EUR 40
Description: Available in various conjugation types.

DNA Staining Reagent

abx299713-100g 100 µg Ask for price

DNA Staining Reagent

abx299713-20g 20 µg
EUR 300

DNA Staining Reagent

abx299713-50g 50 µg Ask for price

Cell Staining Buffer

E-CK-A107-each each Ask for price

Cell Staining Buffer

MBS679239-500mL 500mL
EUR 185

Cell Staining Buffer

MBS679239-5x500mL 5x500mL
EUR 675

Cell Staining Buffer

MBS679240-100mL 100mL
EUR 130

Cell Staining Buffer

MBS679240-5x100mL 5x100mL
EUR 435

Cell Staining Buffer

MBS679241-250mL 250mL
EUR 145

Cell Staining Buffer

MBS679241-5x250mL 5x250mL
EUR 515

Cell Staining Buffer

MBS2567126-100mL 100mL
EUR 90

Cell Staining Buffer

MBS2567126-200mL 200mL
EUR 100

Cell Staining Buffer

MBS2567126-500mL 500mL
EUR 125

Cell Staining Buffer

MBS2567126-5x500mL 5x500mL
EUR 545

DAPI Staining Solution

MBS176580-10mL 10mL
EUR 115

DAPI Staining Solution

MBS176580-50mL 50mL
EUR 135

DAPI Staining Solution

MBS176580-5x50mL 5x50mL
EUR 450

Wright Staining Solution

K1182-100 100ml
EUR 40
Description: Cell Biology|Staining Solution

Wright Staining Solution

K1182-500 500ml
EUR 64
Description: Cell Biology|Staining Solution

ELVIS ID Staining Kit

SK-ELVIS-100 100 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-1000 1000 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-200 200 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-500 500 Tests Ask for price

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 276

Hoechst 33342 Staining Kit

abx097207-100l 100 µl
EUR 162.5

Hoechst 33342 Staining Kit

abx097207-1ml 1 ml Ask for price

Hoechst 33342 Staining Kit

abx097207-200l 200 µl Ask for price

Hoechst 33342 Staining Kit

MBS826727-01mL 0.1mL
EUR 140

Hoechst 33342 Staining Kit

MBS826727-5x01mL 5x0.1mL
EUR 550

Leishman Staining Solution

68015 250 ml
EUR 2.05
Description: Part A

DNA STAINING AGENT (1ML)

NOVELJUICE each
EUR 80

Hematoxylin Staining Buffer

E-IR-R120-15mL 15mL
EUR 60

Hematoxylin Staining Buffer

E-IR-R120-50mL 50mL
EUR 170

Hematoxylin Staining Buffer

E-IR-R120-5mL 5mL
EUR 25

Hematoxylin Staining Buffer

E-IR-R120-each each Ask for price

Hematoxylin staining Buffer

MBS2567138-15mL 15mL
EUR 125

Hematoxylin staining Buffer

MBS2567138-50mL 50mL
EUR 190

Hematoxylin staining Buffer

MBS2567138-5mL 5mL
EUR 95

Hematoxylin staining Buffer

MBS2567138-5x50mL 5x50mL
EUR 815

Cartilage Staining Solution

MBS355813-100mL 100mL
EUR 185

Cartilage Staining Solution

MBS355813-5x100mL 5x100mL
EUR 530

Cell Cycle Staining Kit

KTA2020-each each Ask for price

Cell Cycle Staining Kit

KTA2020-100T 100 T
EUR 119
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-50T 50 T
EUR 69
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

MBS9718793-100Tests 100Tests
EUR 225

Cell Cycle Staining Kit

MBS9718793-500Tests 500Tests
EUR 355

Cell Cycle Staining Kit

MBS9718793-50Tests 50Tests
EUR 195

Cell Cycle Staining Kit

MBS9718793-5x500Tests 5x500Tests
EUR 1580

HISTOCHEMISTRY STAINING TRAY

ATC-12 12 PLACE - CLEAR LID
EUR 226.1

HISTOCHEMISTRY STAINING TRAY

ATC-24 24 PLACE - CLEAR LID
EUR 342.21

HISTOCHEMISTRY STAINING TRAY

AT-12 12 PLACE - BLACK LID
EUR 226.1

HISTOCHEMISTRY STAINING TRAY

AT-24 24 PLACE - BLACK LID
EUR 342.21

Exosome fluorescent staining

S104 -
EUR 800

PI / RNase Staining Buffer

20-abx090619
  • Ask for price
  • Ask for price
  • 100 tests
  • 50 tests

Live NucRed staining kit

EGY069 >100T
EUR 210

PI / RNase Staining Buffer

abx090619-100l 100 µl
EUR 162.5

PI / RNase Staining Buffer

abx090619-1ml 1 ml Ask for price

PI / RNase Staining Buffer

abx090619-200l 200 µl
EUR 200

Live NucRed staining kit

MBS8584964-100Tests 100Tests
EUR 310

Live NucRed staining kit

MBS8584964-5x100Tests 5x100Tests
EUR 1405

PI / RNase Staining Buffer

MBS355225-100Tests 100Tests
EUR 255

PI / RNase Staining Buffer

MBS355225-50Tests 50Tests
EUR 215

PI / RNase Staining Buffer

MBS355225-5x100Tests 5x100Tests
EUR 845

Stains All Gel Staining Kit

KT-792 20 tests
EUR 737

7-AAD Staining Solution

MBS669126-200Tests 200Tests
EUR 145

7-AAD Staining Solution

MBS669126-5x200Tests 5x200Tests
EUR 655

Live NucGreen staining kit

EGY068 >100T
EUR 210

Dead NucGreen staining kit

EGY070 >100T
EUR 210

Live NucGreen staining kit

MBS8584963-100Tests 100Tests
EUR 310

Live NucGreen staining kit

MBS8584963-5x100Tests 5x100Tests
EUR 1405

Dead NucGreen staining kit

MBS8584965-100Tests 100Tests
EUR 310

Dead NucGreen staining kit

MBS8584965-5x100Tests 5x100Tests
EUR 1405

GEL STAINING/INCUBATION BOX

SB-10 LIGHT SHIELDING
EUR 94.44

Ponceau S Staining Solution

41640009-1 500 mL
EUR 40.13

Ponceau S Staining Solution

41640009-2 1 L
EUR 58.16

Ponceau S Staining Solution

B7778-100000 100 g
EUR 78
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes

Ponceau S Staining Solution

B7778-50000 50 g
EUR 53
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes

Ponceau S Staining Solution

14092 100 ml
EUR 13.13
Description: Part B

Ponceau S Staining Solution

EZWB08-1x-100mL 100mL
EUR 9.6

Ponceau S Staining Solution

EZWB08-1x-500mL 500mL
EUR 32.4

Giemsa Staining Solution (10X)

K1181-100 100ml
EUR 40
Description: Cell Biology|Staining Solution

Giemsa Staining Solution (10X)

K1181-500 500ml
EUR 64
Description: Cell Biology|Staining Solution

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 58

Ponceau S Staining Solution

PW005 100ml
EUR 82.97

Ponceau S Staining Solution

MBS355693-100mL 100mL
EUR 180

Ponceau S Staining Solution

MBS355693-5x100mL 5x100mL
EUR 500

Sirius Red Staining Solution

MBS355815-100mL 100mL
EUR 195

Sirius Red Staining Solution

MBS355815-5x100mL 5x100mL
EUR 570

AO/PI Double Staining Kit

K2238-100 100T
EUR 40
Description: Cell Biology|Cell Apoptosis

AO/PI Double Staining Kit

K2238-500 500T
EUR 120
Description: Cell Biology|Cell Apoptosis

Neutral Red Staining Solution

K1185-100 100ml
EUR 40
Description: Cell Biology|Staining Solution

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 101.58

CellSurface FLOW Staining Kit

MBS668926-1Kit 1Kit
EUR 200

CellSurface FLOW Staining Kit

MBS668926-5x1Kit 5x1Kit
EUR 900

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 494.4

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 390.96

PI Viability Staining Solution

E16FXP002 150 tests
EUR 40
Description: Available in various conjugation types.

β-Galactosidase Staining Kit

AKR-100 1 kit
EUR 345

Methyl Green Staining Solution

K1180-100 100ml
EUR 40
Description: Cell Biology|Staining Solution

Methyl Green Staining Solution

abx090698-100l 100 µl
EUR 125

Methyl Green Staining Solution

abx090698-1ml 1 ml Ask for price

Methyl Green Staining Solution

abx090698-200l 200 µl
EUR 187.5

Methyl Green Staining Solution

MBS355816-100mL 100mL
EUR 220

Methyl Green Staining Solution

MBS355816-10mL 10mL
EUR 180

Methyl Green Staining Solution

MBS355816-5x100mL 5x100mL
EUR 685

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 500
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 314.4

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 300
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Lacto Fuchsin Staining Solution

97513 100 ml
EUR 3.01
Description: Part A

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 208.8

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 203

Crystal Violet Staining Solution

K1184-100 100ml
EUR 40
Description: Cell Biology|Staining Solution

Crystal Violet Staining Solution

K1184-500 500ml
EUR 64
Description: Cell Biology|Staining Solution

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 552

Live-Dead Cell Staining Kit

K2081-1000 1000T
EUR 112

Live-Dead Cell Staining Kit

K2081-500 500T
EUR 68

Live-Dead Cell Staining Kit

GWB-AXR262 100 stainings Ask for price

EZ-Quick Slide Staining Set

IW-2510 -
EUR 425

Live-Dead Cell Staining Kit

K501-100 each
EUR 561.6

Human Histochemistry Staining Kit

20-abx299039
  • Ask for price
  • Ask for price
  • Ask for price
  • 150 tests
  • 500 tests
  • 50 tests

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 274

Beta-Galactosidase Staining Kit

K802-250 each
EUR 352.8

Beta-Galactosidase Staining Kit

GWB-AXR372 200 assays. Ask for price

beta-Galactosidase Staining Kit

MBS168046-1Kit 1Kit
EUR 505

beta-Galactosidase Staining Kit

MBS168046-5x1Kit 5x1Kit
EUR 2320

jetBlue Protein Staining Solution

MBS402061-20mL 20mL
EUR 165

jetBlue Protein Staining Solution

MBS402061-500mL 500mL
EUR 210

Nerve Terminal Staining Kit I

70030-1 KT
EUR 605

Nerve Terminal Staining Kit V

70034 1SET
EUR 605
Description: N/A

Nerve Terminal Staining Kit V

70034-1 KT
EUR 605

Purple-Color AP Staining Kit

AP100P-1 100 Assays
EUR 235

FDA Staining Solution (500 tests)

LBF23211 each
EUR 194.02

Slides Staining Jar, Schifferdecker

CG146-5X1NO 1 unit
EUR 53.59
Description: Slides Staining Jar, Schifferdecker

Nerve Terminal Staining Kit IIA

70031-1 1SET
EUR 322
Description: N/A

Nerve Terminal Staining Kit IIB

70031-1-1 KT
EUR 395

Nerve Terminal Staining Kit III

70032-1 KT
EUR 513

Ponceau S Staining Solution, 10×

EZWB08-10x-10mL 10mL
EUR 9

Ponceau S Staining Solution, 10×

EZWB08-10x-50mL 50mL
EUR 28.8

QUANTOM Total Cell Staining Kit

LBQ13501 each
EUR 537.92

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 418
Description: Detects activated Caspases by FACS, FL or plate reader

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 285.6

Manual Staining System (12 Wells)

IMS001 1 ea.
EUR 246.09

Colloidal Protein Staining Solution

51581 100 ml
EUR 25.5
Description: Part B

CrystalStain, Cell Staining Solution

CSS100 100 ml
EUR 9.9
Description: NutriCulture CrystalStain, Cell Staining Solution is a frequently used reagent to visualize cells in cell culture applications like single cell colony assay, Transwell migration assay or Transwell invasion assay.

CrystalStain, Cell Staining Solution

CSS50 50 ml
EUR 6.6
Description: NutriCulture CrystalStain, Cell Staining Solution is a frequently used reagent to visualize cells in cell culture applications like single cell colony assay, Transwell migration assay or Transwell invasion assay.

Lipid (Oil Red O) staining Kit

K580-24 each
EUR 542.4

QUANTOM Viable Cell Staining Kit

LBQ13502 each
EUR 670.35

DNA Ploidy Analysis Staining Kit

K1439-1 each
EUR 705.6

DNA Ploidy Analysis Staining Kit

K1439-5 each
EUR 2402.4

Novel Green DNA Staining Reagent

abx299721-100g 100 µg Ask for price

Novel Green DNA Staining Reagent

abx299721-20g 20 µg
EUR 325

Novel Green DNA Staining Reagent

abx299721-50g 50 µg Ask for price

Prime Juice DNA Staining Reagent

LD011-1000 1 ml
EUR 10

Propidium Iodide (PI) Staining Kit

abx097208-100l 100 µl
EUR 162.5

However, the have an effect on of ART on the nicely being of every victims and their offspring continues to set off concern, and many ethical challenges created by new scientific developments in this space entice extensively differing opinions.

What is apparent is that there may be a sustained world growth in utilisation of ART and that reproductive tourism will allow many people to entry the remedy they want nevertheless nationwide legal guidelines that may forbid some approaches. The greatest drawback is to broaden entry to ART to those residing in the a lot much less wealthy nations who’re equally deserving of its benefits.