Study protocol: E-freeze – freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

Infertility impacts one in seven {{couples}}; many of these need in vitro fertilisation (IVF). IVF entails exterior hormones to stimulate a girl’s ovaries to produce eggs which might be harvested surgically. Embryos, created in the laboratory by mixing eggs with sperm, are grown in custom for a few days sooner than being modified inside the uterus (fresh embryo transfer).

Spare embryos are sometimes frozen with a view to transfer at a later degree in time – significantly if the preliminary fresh transfer does not consequence in a being pregnant. Despite enhancements in know-how, IVF success costs keep low with an complete reside starting value of 25-30% per remedy. Additionally, there are issues about nicely being outcomes for mothers and infants conceived by IVF, notably after fresh embryo transfer, collectively with maternal ovarian hyperstimulation syndrome (OHSS) and preterm provide.

It is believed that prime ranges of hormones all through ovarian stimulation could create a comparatively hostile setting for embryo implantation whereas rising the menace of OHSS. It has been steered that freezing all embryos with the intention of thawing and altering them inside the uterus at a later stage (thawed frozen embryo transfer) as a substitute of fresh embryo transfer, would possibly outcome in improved being pregnant costs and fewer issues. We goal to examine the clinical and cost effectiveness of fresh and thawed frozen embryo transfer, with the main goal of determining any distinction in the likelihood of having a healthful baby.

E-Freeze is a pragmatic, multicentre two-arm parallel group randomised controlled trial the place women aged ≥18 and < 42 years, with not lower than three good prime quality embryos are randomly allotted to acquire each a fresh or thawed frozen embryo transfer.

The main remaining result’s a healthful baby, outlined as a time interval, singleton, reside starting with relevant weight for gestation. Cost effectiveness may be calculated from a healthcare and societal perspective.E-Freeze will determine the relative benefits of fresh and thawed frozen embryo transfer in phrases of enhancing the likelihood of having a healthful baby. The outcomes of this pragmatic study have the potential to be straight transferred to clinical observe.ISRCTN registry

 Study protocol: E-freeze - freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

Study protocol: E-freeze – freezing of embryos in assisted conception: a randomised controlled trial evaluating the clinical and cost effectiveness of a policy of freezing embryos followed by thawed frozen embryo transfer compared with a policy of fresh embryo transfer, in women undergoing in vitro fertilisation.

In vitro fertilisation/intracytoplasmic sperm injection previous 2020.

Over eight million infants have been born following IVF (in vitro fertilisation) and completely different artificial reproductive know-how (ART) procedures since Louise Brown’s starting 40 years in the previous. New enhancements have added a lot complexity to every clinical and laboratory procedures over the remaining four a few years. Translation of novel approaches from major science into clinical observe continues unabated, widening the applicability of ART to new groups of people and serving to boost every chances of healthful reside starting and affected particular person acceptability.

STAT Double Staining Reagent for Double staining o

NB327DB 25 ml
EUR 595
Description: STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 276

HE Staining Kit

E-IR-R117-100mL 100mL
EUR 240

HE Staining Kit

E-IR-R117-10mL 10mL
EUR 35

HE Staining Kit

E-IR-R117-50mL 50mL
EUR 150

HE Staining Kit

E-IR-R117-each each Ask for price

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

TRAP Staining Kit

KT-008 1 kit
EUR 747

DAPI Staining Kit

abx097206-100l 100 µl
EUR 162.5

DAPI Staining Kit

abx097206-1ml 1 ml Ask for price

DAPI Staining Kit

abx097206-200l 200 µl Ask for price

Coplin Staining Jar

08415-3 3sets
EUR 84

Silver Staining Kit

ML123-1KT 1 unit
EUR 201.16
Description: Silver Staining Kit

Cell Staining Buffer

E-CK-A107-100mL 100 mL
EUR 20

Cell Staining Buffer

E-CK-A107-200mL 200 mL
EUR 35

Cell Staining Buffer

E-CK-A107-500mL 500 mL
EUR 60

Cell Staining Buffer

E16FXP005 500 ml
EUR 40
Description: Available in various conjugation types.

DNA Staining Reagent

abx299713-100g 100 µg Ask for price

DNA Staining Reagent

abx299713-20g 20 µg
EUR 300

DNA Staining Reagent

abx299713-50g 50 µg Ask for price

Cell Staining Buffer

E-CK-A107-each each Ask for price

ELVIS ID Staining Kit

SK-ELVIS-100 100 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-1000 1000 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-200 200 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-500 500 Tests Ask for price

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 276

Hoechst 33342 Staining Kit

abx097207-100l 100 µl
EUR 162.5

Hoechst 33342 Staining Kit

abx097207-1ml 1 ml Ask for price

Hoechst 33342 Staining Kit

abx097207-200l 200 µl Ask for price

DNA STAINING AGENT (1ML)

NOVELJUICE each
EUR 80

Cell Cycle Staining Kit

KTA2020-100T 100 T
EUR 119
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-50T 50 T
EUR 69
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-each each Ask for price

Hematoxylin Staining Buffer

E-IR-R120-15mL 15mL
EUR 60

Hematoxylin Staining Buffer

E-IR-R120-50mL 50mL
EUR 170

Hematoxylin Staining Buffer

E-IR-R120-5mL 5mL
EUR 25

Hematoxylin Staining Buffer

E-IR-R120-each each Ask for price

Live NucRed staining kit

EGY069 >100T
EUR 210

PI / RNase Staining Buffer

20-abx090619
  • Ask for price
  • Ask for price
  • 100 tests
  • 50 tests

PI / RNase Staining Buffer

abx090619-100l 100 µl
EUR 162.5

PI / RNase Staining Buffer

abx090619-1ml 1 ml Ask for price

PI / RNase Staining Buffer

abx090619-200l 200 µl
EUR 200

HISTOCHEMISTRY STAINING TRAY

ATC-12 12 PLACE - CLEAR LID
EUR 226.1

HISTOCHEMISTRY STAINING TRAY

ATC-24 24 PLACE - CLEAR LID
EUR 342.21

HISTOCHEMISTRY STAINING TRAY

AT-12 12 PLACE - BLACK LID
EUR 226.1

HISTOCHEMISTRY STAINING TRAY

AT-24 24 PLACE - BLACK LID
EUR 342.21

Exosome fluorescent staining

S104 -
EUR 800

Stains All Gel Staining Kit

KT-792 20 tests
EUR 737

Live NucGreen staining kit

EGY068 >100T
EUR 210

Dead NucGreen staining kit

EGY070 >100T
EUR 210

GEL STAINING/INCUBATION BOX

SB-10 LIGHT SHIELDING
EUR 94.44

Ponceau S Staining Solution

41640009-1 500 mL
EUR 40.13

Ponceau S Staining Solution

41640009-2 1 L
EUR 58.16

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 58

Ponceau S Staining Solution

B7778-100000 100 g
EUR 78
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes

Ponceau S Staining Solution

B7778-50000 50 g
EUR 53
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes

Ponceau S Staining Solution

PW005 100ml
EUR 82.97

Ponceau S Staining Solution

EZWB08-1x-100mL 100mL
EUR 9.6

Ponceau S Staining Solution

EZWB08-1x-500mL 500mL
EUR 32.4

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 494.4

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 101.58

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 500
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 314.4

PI Viability Staining Solution

E16FXP002 150 tests
EUR 40
Description: Available in various conjugation types.

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 390.96

β-Galactosidase Staining Kit

AKR-100 1 kit
EUR 345

Methyl Green Staining Solution

abx090698-100l 100 µl
EUR 125

Methyl Green Staining Solution

abx090698-1ml 1 ml Ask for price

Methyl Green Staining Solution

abx090698-200l 200 µl
EUR 187.5

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 203

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 300
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 208.8

Live-Dead Cell Staining Kit

GWB-AXR262 100 stainings Ask for price

Live-Dead Cell Staining Kit

K501-100 each
EUR 561.6

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 552

Live-Dead Cell Staining Kit

K2081-1000 1000T
EUR 112

Live-Dead Cell Staining Kit

K2081-500 500T
EUR 68

EZ-Quick Slide Staining Set

IW-2510 -
EUR 425

Nerve Terminal Staining Kit I

70030-1 KT
EUR 605

Nerve Terminal Staining Kit V

70034 1SET
EUR 605
Description: N/A

Nerve Terminal Staining Kit V

70034-1 KT
EUR 605

Purple-Color AP Staining Kit

AP100P-1 100 Assays
EUR 235

Human Histochemistry Staining Kit

20-abx299039
  • Ask for price
  • Ask for price
  • Ask for price
  • 150 tests
  • 500 tests
  • 50 tests

Beta-Galactosidase Staining Kit

GWB-AXR372 200 assays. Ask for price

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 274

Beta-Galactosidase Staining Kit

K802-250 each
EUR 352.8

Slides Staining Jar, Schifferdecker

CG146-5X1NO 1 unit
EUR 53.59
Description: Slides Staining Jar, Schifferdecker

Lipid (Oil Red O) staining Kit

K580-24 each
EUR 542.4

Nerve Terminal Staining Kit IIA

70031-1 1SET
EUR 322
Description: N/A

Nerve Terminal Staining Kit IIB

70031-1-1 KT
EUR 395

Nerve Terminal Staining Kit III

70032-1 KT
EUR 513

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 418
Description: Detects activated Caspases by FACS, FL or plate reader

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 285.6

Manual Staining System (12 Wells)

IMS001 1 ea.
EUR 246.09

Ponceau S Staining Solution, 10×

EZWB08-10x-10mL 10mL
EUR 9

Ponceau S Staining Solution, 10×

EZWB08-10x-50mL 50mL
EUR 28.8

DNA Ploidy Analysis Staining Kit

K1439-1 each
EUR 705.6

DNA Ploidy Analysis Staining Kit

K1439-5 each
EUR 2402.4

Prime Juice DNA Staining Reagent

LD011-1000 1 ml
EUR 10

Novel Green DNA Staining Reagent

abx299721-100g 100 µg Ask for price

Novel Green DNA Staining Reagent

abx299721-20g 20 µg
EUR 325

Novel Green DNA Staining Reagent

abx299721-50g 50 µg Ask for price

Propidium Iodide (PI) Staining Kit

abx097208-100l 100 µl
EUR 162.5

Propidium Iodide (PI) Staining Kit

abx097208-1ml 1 ml Ask for price

Propidium Iodide (PI) Staining Kit

abx097208-200l 200 µl Ask for price

DAPI staining kit (ready to use) 

EGY0621 10mL
EUR 85

DAPI staining kit (ready to use) 

EGY0622 50mL
EUR 185

Alcian Blue (pH 2.5) Staining Kit

AFR-1 250 ml ea.
EUR 127.28

Alcian Blue (pH 2.5) Staining Kit

AFR-2 30 ml ea.
EUR 63

7-AAD Viability Staining Buffer

abx090617-100tests 100 tests
EUR 260.4

Plastic Coplin Staining Jars, each

IW-2501 -
EUR 8.6

7-AAD Viability Staining Buffer

abx090617-100l 100 µl
EUR 150

7-AAD Viability Staining Buffer

abx090617-1ml 1 ml Ask for price

7-AAD Viability Staining Buffer

abx090617-200l 200 µl Ask for price

Stayrightâ„¢ Purple HRP Staining Kit

45905 5 mL
EUR 112
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45905-5mL 5 mL
EUR 109
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45906 50 mL
EUR 341
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45906-50mL 50 mL
EUR 334
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Hematoxylin and Eosin Staining Kit

K1142-1000 2×500mL
EUR 62
Description: Hematoxylin and Eosin Staining,one of the most widely used staining technique in histopathology

TRAP Staining Kit with Osteoplates

KT-651 1 kit
EUR 1003

Hochst33342/PI Double Staining Kit

E37F032-100-tests 100tests
EUR 66.67
Description: Double staining kit

PAP PEN FOR IMMUNO STAINING - EACH

Z377821-1EA EACH
EUR 192.41

7-AAD Viability Staining Solution

E16FXP003 150 tests
EUR 145
Description: Available in various conjugation types.

7-AAD Viability Staining Solution

EXB0026 400 tests
EUR 44

Nalgene Box Staining 13x13x5cm - EACH

BOX1040 EACH
EUR 54

SafePinky DNA Gel Staining Solution

S1000-050 500ml
EUR 157.2

SafePinky DNA Gel Staining Solution

S1000-100 2X500ml
EUR 225.6

SafePinky DNA Gel Staining Solution

S1000-200 4X500ml
EUR 336

Disposable Slide Staining Trays - PK4

MIC5070 PK4
EUR 101.25

Slides Staining Jar, Hellendhal Type

CG145-5X1NO 1 unit
EUR 61.59
Description: Slides Staining Jar, Hellendhal Type

Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
EUR 292.8

Wheaton staining dishes 3 EA - PK3

S5017-3EA PK3
EUR 152.55

Calcein AM/PI Double Staining Kit

E-CK-A354-each each Ask for price
Description: Viability/Cytotoxicity

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 613
Description: Caspase Staining Kit for use activity in the research laboratory

Staining Trough+Lid Rectangular - EACH

MIC6008 EACH
EUR 31.05

Ponceau S Protein Staining Solution

PS003-B500ML 500 ml
EUR 25

Mouse to Mouse HRP Staining System

MTM003 400 Slides
EUR 249.43

Slides Staining Jar, with Ground - in

CG147-5X1NO 1 unit
EUR 124
Description: Slides Staining Jar, with Ground - in

Preloading Fluorescent Staining Solution

abx460011-1096tests 10 × 96 tests Ask for price

Preloading Fluorescent Staining Solution

abx460011-596tests 5 × 96 tests Ask for price

Preloading Fluorescent Staining Solution

abx460011-96tests 96 tests
EUR 250

Staining Jar PP + Lid 10 Slide - EACH

MIC6002 EACH
EUR 75.6

Coomassie Blue Fast Staining Solution

AR0170 100mL(for 10 assays to stain gels of 5 x 8.5cm)
EUR 96

Alkaline Phosphatase Red Staining kit

TBS2080 50 tests
EUR 89

Coomassie Blue Fast Staining Solution

EZWB15-500mL 500mL
EUR 14.4

Calcein-AM/PI Double Staining Kit

E-CK-A354-100Assays 100 Assays
EUR 100

Calcein-AM/PI Double Staining Kit

E-CK-A354-2000Assays 2000 Assays
EUR 600

Calcein-AM/PI Double Staining Kit

E-CK-A354-500Assays 500 Assays
EUR 300

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 610
Description: Detects activated Caspases-3 by FACS, FL or plate reader

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 285.6

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 490
Description: Detects activated Caspases-8 by FACS, FL or plate reader

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 285.6

Red Active Caspase-9 Staining Kit

K2055-100 100 assays
EUR 610
Description: Detects activated Caspases-9 by FACS, FL or plate reader

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 285.6

Slides Staining Racks for 30 pieces,

CG149-5X1NO 1 unit
EUR 124
Description: Slides Staining Racks for 30 pieces,

Calcein-AM/PI Double Staining Kit

E37F030-500-tests 500tests
EUR 266.67
Description: Double staining kit

Calcein-AM/PI Double Staining Kit

E37F031-1000-tests 1000tests
EUR 526.67
Description: Double staining kit

Trypan Blue Staining Cell Assay Kit

APB0003-10 5ml
EUR 0.1

Alkaline Phosphatase Blue Staining kit

TBS2085 50 tests
EUR 89

OOSB00015-500ML - Cell Staining Buffer

OOSB00015-500ML 500mL
EUR 119

OOSB00016-250ML - Cell Staining Buffer

OOSB00016-250ML 250mL
EUR 69

OOSB00017-100ML - Cell Staining Buffer

OOSB00017-100ML 100mL
EUR 39

OOTA00782-500ML - Flow Staining Buffer

OOTA00782-500ML 500mL
EUR 79

Four color suit nuclear staining Kit

EGY071 100T
EUR 1190

EasyDip Slide Staining Jar - Blue, each

M900-12B -
EUR 16.98

EasyDip Slide Staining Jar - Pink, each

M900-12P -
EUR 16.98

Staining Jar W Lid 115x115x77mm - PK10

MIC6016 PK10
EUR 481.95

Hoechst 33342 staining kit (ready to use) 

EGY0631 10mL
EUR 50

Hoechst 33342 staining kit (ready to use) 

EGY0632 50mL
EUR 160

Hoechst 33258 staining kit (ready to use) 

EGY0641 10mL
EUR 50

Hoechst 33258 staining kit (ready to use) 

EGY0642 50mL
EUR 145

Foxp3/Transcription Factor Staining Kit

E-CK-A108-20Assays 20 Assays
EUR 75

Fluorescein Active Caspase Staining Kit

K2047-100 100 assays
EUR 610
Description: Detects activated Caspases by FACS, FL or plate reader

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 285.6

Foxp3/Transcription Factor Staining Kit

E-CK-A108-each each Ask for price

Yeast Viability Staining Kit (Red/Blue)

31063-2-1 KT
EUR 475

Human Type I Collagen Staining Other

9044 1 kit
EUR 233
Description: Tissue Slides, Cultured Cells

EasyDip Slide Staining Jar - Green, each

M900-12G -
EUR 16.98

EasyDip Slide Staining Jar - White, each

M900-12W -
EUR 16.98

However, the have an effect on of ART on the nicely being of every victims and their offspring continues to set off concern, and many ethical challenges created by new scientific developments in this space entice extensively differing opinions.

What is apparent is that there may be a sustained world growth in utilisation of ART and that reproductive tourism will allow many people to entry the remedy they want nevertheless nationwide legal guidelines that may forbid some approaches. The greatest drawback is to broaden entry to ART to those residing in the a lot much less wealthy nations who’re equally deserving of its benefits.